TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage.
Animals
Animals, Newborn
Female
Gene Expression Regulation
Mice
Mice, Inbred C57BL
Mutation
/ genetics
NIH 3T3 Cells
Nuclear Proteins
/ metabolism
Oocytes
/ metabolism
Promoter Regions, Genetic
RNA, Messenger
/ genetics
TATA Box
Terminal Repeat Sequences
/ genetics
Transcription Factor TFIIA
/ metabolism
Transcription Factor TFIID
/ metabolism
Transcription, Genetic
Transcriptome
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
22 12 2020
22 12 2020
Historique:
received:
22
09
2020
accepted:
20
11
2020
entrez:
23
12
2020
pubmed:
24
12
2020
medline:
14
1
2021
Statut:
epublish
Résumé
During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses demonstrate that TBPL2 mediates transcription of oocyte-expressed genes, including mRNA survey genes, as well as specific endogenous retroviral elements. Transcription start site (TSS) mapping indicates that TBPL2 has a strong preference for TATA-like motif in core promoters driving sharp TSS selection, in contrast with canonical TBP/TFIID-driven TATA-less promoters that have broader TSS architecture. Thus, we show a role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.
Identifiants
pubmed: 33353944
doi: 10.1038/s41467-020-20239-4
pii: 10.1038/s41467-020-20239-4
pmc: PMC7755920
doi:
Substances chimiques
Nuclear Proteins
0
RNA, Messenger
0
Transcription Factor TFIIA
0
Transcription Factor TFIID
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6439Subventions
Organisme : Medical Research Council
ID : MC_UP_1102/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 106115/Z/14/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 106955/Z/15/Z
Pays : United Kingdom
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