EBV DNA increase in COVID-19 patients with impaired lymphocyte subpopulation count.


Journal

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 04 11 2020
revised: 10 12 2020
accepted: 17 12 2020
pubmed: 29 12 2020
medline: 5 5 2021
entrez: 28 12 2020
Statut: ppublish

Résumé

The immunologic profile and opportunistic viral DNA increase were monitored in Italian patients with COVID-19 in order to identify markers of disease severity. A total of 104 patients infected with SARS-CoV-2 were evaluated in the study. Of them, 42/104 (40.4%) were hospitalized in an intensive care unit (ICU) and 62/104(59.6%) in a sub-intensive care unit (SICU). Human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), Parvovirus B19 and Human Herpesvirus 6 virus reactivations were determined by real-time PCR, and lymphocyte subpopulation counts were determined by flow cytometry. Among opportunistic viruses, only EBV was consistently detected. EBV DNA was observed in 40/42 (95.2%) of the ICU patients and in 51/61 (83.6%) of the SICU patients. Comparing the two groups of patients, the EBV DNA median level among ICU patients was significantly higher than that observed in SICU patients. In parallel, a significant reduction of CD8 T cell and NK count in ICU patients as compared with SICU patients was observed (p<0.05). In contrast, B cell count was significantly increased in ICU patients (p=0.0172). A correlation between reduced CD8

Identifiants

pubmed: 33359064
pii: S1201-9712(20)32575-3
doi: 10.1016/j.ijid.2020.12.051
pmc: PMC7833117
pii:
doi:

Substances chimiques

DNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

315-319

Investigateurs

R Bruno (R)
M Mondelli (M)
E Brunetti (E)
A Di Matteo (A)
E Seminari (E)
L Maiocchi (L)
V Zuccaro (V)
L Pagnucco (L)
B Mariani (B)
S Ludovisi (S)
R Lissandrin (R)
A Parisi (A)
P Sacchi (P)
Sfa Patruno (S)
G Michelone (G)
R Gulminetti (R)
D Zanaboni (D)
S Novati (S)
R Maserati (R)
P Orsolini (P)
M Vecchia (M)
M Sciarra (M)
E Asperges (E)
M Colaneri (M)
A Di Filippo (A)
M Sambo (M)
S Biscarini (S)
M Lupi (M)
S Roda (S)
T C Pieri (TC)
I Gallazzi (I)
M Sachs (M)
P Valsecchi (P)
S Perlini (S)
C Alfano (C)
M Bonzano (M)
F Briganti (F)
G Crescenzi (G)
A G Falchi (AG)
R Guarnone (R)
B Guglielmana (B)
E Maggi (E)
I Martino (I)
P Pettenazza (P)
S Pioli di Marco (S)
F Quaglia (F)
A Sabena (A)
F Salinaro (F)
F Speciale (F)
I Zunino (I)
M De Lorenzo (M)
G Secco (G)
L Dimitry (L)
G Cappa (G)
I Maisak (I)
B Chiodi (B)
M Sciarrini (M)
B Barcella (B)
F Resta (F)
L Moroni (L)
G Vezzoni (G)
L Scattaglia (L)
E Boscolo (E)
C Zattera (C)
M F Tassi (MF)
V Capozza (V)
D Vignaroli (D)
M Bazzini (M)
G Iotti (G)
F Mojoli (F)
M Belliato (M)
L Perotti (L)
S Mongodi (S)
G Tavazzi (G)
G Marseglia (G)
A Licari (A)
I Brambilla (I)
D Barbarini (D)
A Bruno (A)
P Cambieri (P)
G Campanini (G)
C Cavanna (C)
G Comolli (G)
M Corbella (M)
R Daturi (R)
M Furione (M)
B Mariani (B)
P Marone (P)
R Maserati (R)
E Monzillo (E)
S Paolucci (S)
M Parea (M)
E Percivalle (E)
A Piralla (A)
F Rovida (F)
A Sarasini (A)
M Zavattoni (M)
G Adzasehoun (G)
M Ardizzone (M)
L Bellotti (L)
V Brunco (V)
E Cabano (E)
G Casali (G)
L Capella (L)
D Devitis (D)
L Dossena (L)
G Frisco (G)
G Garbagnoli (G)
F Gardellini (F)
A Girello (A)
A Guerrizio (A)
V Landini (V)
C Lucchelli (C)
V Maliardi (V)
P Piemontese (P)
S Pezzaia (S)
M Premoli (M)
C Rebuffa (C)
C Zanello (C)
J Bagnarino (J)
F Bergami (F)
A Bonetti (A)
G Caneva (G)
I Cassaniti (I)
A Corcione (A)
R Di Martino (R)
A Di Napoli (A)
A Ferrari (A)
G Ferrari (G)
L Fiorina (L)
A Gallone (A)
F Giardina (F)
A Girardi (A)
A Mercato (A)
C Merla (C)
F Novazzi (F)
G Ratano (G)
B Rossi (B)
G Saveriaempillai (G)
I M Sciabica (IM)
M Tallarita (M)
E Vecchio Nepita (E)
J Vitali (J)
A Cerino (A)
S Varchetta (S)
B Oliviero (B)
S Mantovani (S)
D Mele (D)
M Calvi (M)
M Tizzoni (M)
C Nicora (C)
A Triarico (A)
V Petronella (V)
C Marena (C)
A Muzzi (A)
P Lago (P)
S Cutti (S)
V Novelli (V)
F Comandatore (F)
G BatistiBiffignandi (G)
S Gaiarsa (S)
M Rettani (M)
C Bandi (C)
A Ferrari (A)

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.

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Auteurs

Stefania Paolucci (S)

Molecular Virology Unit, Microbiology and VirologyDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Irene Cassaniti (I)

Molecular Virology Unit, Microbiology and VirologyDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Federica Novazzi (F)

Molecular Virology Unit, Microbiology and VirologyDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Loretta Fiorina (L)

Laboratorio Genetica - Trapiantologia e Malattie Cardiovascolari, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Antonio Piralla (A)

Molecular Virology Unit, Microbiology and VirologyDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Giuditta Comolli (G)

Molecular Virology Unit, Microbiology and VirologyDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Experimental ResearchLaboratories, Biotechnology Area, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Raffaele Bruno (R)

Infectious DiseaseDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy.

Renato Maserati (R)

Infectious DiseaseDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Roberto Gulminetti (R)

Infectious DiseaseDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Stefano Novati (S)

Infectious DiseaseDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Francesco Mojoli (F)

Anesthesia and Intensive Care, Emergency Department, Fondazione IRCCS Policlinico San Matteo, Pavia, Pavia, Italy; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy.

Fausto Baldanti (F)

Molecular Virology Unit, Microbiology and VirologyDepartment, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. Electronic address: f.baldanti@smatteo.pv.it.

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