Presence of pathogenic Leptospira spp. in the reproductive system and fetuses of wild boars (Sus scrofa) in Italy.
Animals
Bacterial Typing Techniques
Epididymis
/ microbiology
Female
Fetus
/ parasitology
Genotyping Techniques
Italy
/ epidemiology
Leptospira
/ genetics
Leptospira interrogans
/ genetics
Leptospirosis
/ epidemiology
Male
Multilocus Sequence Typing
Placenta
/ microbiology
Pregnancy
Reproductive Tract Infections
/ microbiology
Sus scrofa
/ microbiology
Swine
/ microbiology
Testis
/ microbiology
Uterus
/ microbiology
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
19
05
2020
accepted:
13
11
2020
revised:
08
01
2021
pubmed:
29
12
2020
medline:
17
2
2021
entrez:
28
12
2020
Statut:
epublish
Résumé
Leptospirosis is a re-emerging and globally spread zoonosis caused by pathogenic genomospecies of Leptospira. Wild boar (Sus scrofa) are an important Leptospira host and are increasing in population all over Europe. The aim of this investigation was to evaluate Leptospira spp. infection in the reproductive systems of wild boar hunted in two Italian regions: Tuscany and Sardinia. From 231 animals, reproductive system tissue samples (testicles, epididymides, uteri) as well as placentas and fetuses were collected. Bacteriological examination and Real-Time PCR were performed to detect pathogenic Leptospira (lipL32 gene). Leptospires were isolated from the testicles and epididymides of one adult and two subadult wild boar. Four isolates from the two subadult males were identified as Leptospira interrogans serogroup Australis by MLST, whereas Leptospira kirschneri serogroup Grippotyphosa was identified from the adult testicles and epididymis. Using Real-Time PCR, 70 samples were positive: 22 testicles (23.16%) and 22 epididymides (23.16%), 10 uteri (7.35%), 3 placentas (6.66%), and 13 fetuses (28.88%). Amplification of the rrs2 gene identified L. interrogans and L. kirschneri species. The results from this investigation confirmed that wild boar represent a potential source of pathogenic Leptospira spp. Isolation of Leptospira serogroups Australis and Grippotyphosa from the male reproductive system and the positive Real-Time PCR results from both male and female samples could suggest venereal transmission, as already demonstrated in pigs. Furthermore, placentas and fetuses were positive for the lipL32 target, and this finding may be related to a possible vertical transmission of pathogenic Leptospira.
Identifiants
pubmed: 33370309
doi: 10.1371/journal.pntd.0008982
pii: PNTD-D-20-00871
pmc: PMC7793250
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0008982Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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