Synthesis and Matrix Properties of α-Cyano-5-phenyl-2,4-pentadienic Acid (CPPA) for Intact Proteins Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.
MALDI matrix
bacteria
hazelnut
mass spectrometry
milk
proteomics
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
21 Dec 2020
21 Dec 2020
Historique:
received:
30
10
2020
revised:
14
12
2020
accepted:
15
12
2020
entrez:
29
12
2020
pubmed:
30
12
2020
medline:
10
4
2021
Statut:
epublish
Résumé
The effectiveness of a synthesized matrix, α-cyano-5-phenyl-2,4-pentadienic acid (CPPA), for protein analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in complex samples such as foodstuff and bacterial extracts, is demonstrated. Ultraviolet (UV) absorption along with laser desorption/ionization mass spectrometry (LDI-MS) experiments were systematically conducted in positive ion mode under standard Nd:YLF laser excitation with the aim of characterizing the matrix in terms of wavelength absorption and proton affinity. Besides, the results for standard proteins revealed that CPPA significantly enhanced the protein signals, reduced the spot-to-spot variability and increased the spot homogeneity. The CPPA matrix was successful employed to investigate intact microorganisms, milk and seed extracts for protein profiling. Compared to conventional matrices such as sinapinic acid (SA), α-cyano-4-hydroxycinnamic acid (CHCA) and 4-chloro-α-cyanocinnamic acid (CClCA), CPPA exhibited better signal-to-noise (S/N) ratios and a uniform response for most examined proteins occurring in milk, hazelnut and in intact bacterial cells of
Identifiants
pubmed: 33371472
pii: molecules25246054
doi: 10.3390/molecules25246054
pmc: PMC7767571
pii:
doi:
Substances chimiques
4-chloro-alpha-cyanocinnamic acid
0
Cinnamates
0
Coumaric Acids
0
Proteins
0
alpha-cyano-4-hydroxycinnamate
28166-41-8
sinapinic acid
68A28V6010
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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