Bisphosphonates Reduce Smoking-Induced Osteoporotic-Like Alterations by Regulating RANKL/OPG in an Osteoblast and Osteoclast Co-Culture Model.
Bone Density Conservation Agents
/ pharmacology
Cell Differentiation
Cell Line, Tumor
Cell Survival
/ drug effects
Coculture Techniques
Diphosphonates
/ pharmacology
Dose-Response Relationship, Drug
Gene Expression Regulation
/ drug effects
Humans
Immunohistochemistry
Osteoblasts
/ drug effects
Osteoclasts
/ drug effects
Osteoporosis
/ etiology
Osteoprotegerin
/ genetics
RANK Ligand
/ genetics
Smoking
/ adverse effects
bisphosphonates
cigarette smoking
co-culture
osteoblasts
osteoclasts
osteoporosis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
23 Dec 2020
23 Dec 2020
Historique:
received:
17
11
2020
accepted:
21
12
2020
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
2
4
2021
Statut:
epublish
Résumé
Co-culture models have become mandatory for obtaining better insights into bone homeostasis, which relies on the balance between osteoblasts and osteoclasts. Cigarette smoking (CS) has been proven to increase the risk of osteoporosis; however, there is currently no proven treatment for osteoporosis in smokers excluding cessation. Bisphosphonates (BPs) are classical anti-osteoclastic drugs that are commonly used in examining the suitability of bone co-culture systems in vitro as well as to verify the response to osteoporotic stimuli. In the present study, we tested the effects of BPs on cigarette smoke extract (CSE)-affected cells in the co-culture of osteoblasts and osteoclasts. Our results showed that BPs were able to reduce CSE-induced osteoporotic alterations in the co-culture of osteoblasts and osteoclasts such as decreased matrix remodeling, enhanced osteoclast activation, and an up-regulated receptor activator of nuclear factor (NF)-kB-ligand (RANKL)/osteoprotegerin (OPG) ratio. In summary, BPs may be an effective alternative therapy for reversing osteoporotic alterations in smokers, and the potential mechanism is through modulation of the RANKL/OPG ratio.
Identifiants
pubmed: 33374546
pii: ijms22010053
doi: 10.3390/ijms22010053
pmc: PMC7793101
pii:
doi:
Substances chimiques
Bone Density Conservation Agents
0
Diphosphonates
0
Osteoprotegerin
0
RANK Ligand
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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