Modulation of Aub-TDRD interactions elucidates piRNA amplification and germplasm formation.
Animals
Animals, Genetically Modified
Arginine
/ metabolism
Argonaute Proteins
/ metabolism
Cytoplasm
/ metabolism
DNA Transposable Elements
/ genetics
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Female
Gene Amplification
Germ Cells
/ metabolism
Male
Ovary
/ metabolism
Peptide Initiation Factors
/ genetics
RNA, Messenger
/ metabolism
RNA, Small Interfering
/ genetics
Tudor Domain
/ genetics
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
22
09
2020
revised:
13
12
2020
accepted:
14
12
2020
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
15
9
2021
Statut:
epublish
Résumé
Aub guided by piRNAs ensures genome integrity by cleaving retrotransposons, and genome propagation by trapping mRNAs to form the germplasm that instructs germ cell formation. Arginines at the N-terminus of Aub (Aub-NTRs) interact with Tudor and other Tudor domain-containing proteins (TDRDs). Aub-TDRD interactions suppress active retrotransposons via piRNA amplification and form germplasm via generation of Aub-Tudor ribonucleoproteins. Here, we show that Aub-NTRs are dispensable for primary piRNA biogenesis but essential for piRNA amplification and that their symmetric dimethylation is required for germplasm formation and germ cell specification but largely redundant for piRNA amplification.
Identifiants
pubmed: 33376130
pii: 4/3/e202000912
doi: 10.26508/lsa.202000912
pmc: PMC7772777
pii:
doi:
Substances chimiques
Argonaute Proteins
0
DNA Transposable Elements
0
Drosophila Proteins
0
Peptide Initiation Factors
0
RNA, Messenger
0
RNA, Small Interfering
0
aub protein, Drosophila
0
piwi protein, Drosophila
0
Arginine
94ZLA3W45F
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM106047
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123512
Pays : United States
Informations de copyright
© 2020 Vrettos et al.
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