Evaluation in Swine of a Recombinant Georgia 2010 African Swine Fever Virus Lacking the I8L Gene.
ASF
ASFV
African swine fever
I8L gene
recombinant ASFV
virus virulence
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
29 12 2020
29 12 2020
Historique:
received:
04
12
2020
revised:
20
12
2020
accepted:
20
12
2020
entrez:
1
1
2021
pubmed:
2
1
2021
medline:
24
2
2021
Statut:
epublish
Résumé
African swine fever virus (ASFV) is the causative agent of African swine fever, a disease currently causing significant economic losses in Europe and Asia. Specifically, the highly virulent ASFV strain Georgia 2010 (ASFV-G) is producing disease outbreaks in this large geographical region. The ASFV genome encodes for over 150 genes, most of which are still not experimentally characterized. I8L is a highly conserved gene that has not been studied beyond its initial description as a virus ORF. Transcriptional analysis of swine macrophages infected with ASFV-G demonstrated that the I8L gene is transcribed early during the virus replication cycle. To assess the importance of I8L during ASFV-G replication in vitro and in vivo, as well as its role in virus virulence in domestic swine, we developed a recombinant virus lacking the I8L gene (ASFV-G-ΔI8L). Replication of ASFV-G-ΔI8L was similar to parental ASFV-G replication in primary swine macrophage cultures, suggesting that the I8L gene is not essential for ASFV-G replication in vitro. Similarly, replication of ASFV-G-ΔI8L in swine intramuscularly inoculated with 10
Identifiants
pubmed: 33383814
pii: v13010039
doi: 10.3390/v13010039
pmc: PMC7823879
pii:
doi:
Substances chimiques
Viral Proteins
0
Types de publication
Historical Article
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Références
J Virol. 2017 Nov 30;91(24):
pubmed: 28978700
Sci Rep. 2017 Apr 24;7:46747
pubmed: 28436458
J Virol. 2015 Jun;89(11):6048-56
pubmed: 25810553
PLoS One. 2019 Nov 14;14(11):e0223955
pubmed: 31725732
Emerg Infect Dis. 2011 Apr;17(4):599-605
pubmed: 21470447
Sci Rep. 2018 Oct 2;8(1):14714
pubmed: 30279544
Sci China Life Sci. 2020 May;63(5):623-634
pubmed: 32124180
Virus Res. 2018 Apr 2;249:116-123
pubmed: 29605728
J Virol. 1998 Apr;72(4):3185-95
pubmed: 9580160
Viruses. 2019 Sep 11;11(9):
pubmed: 31514438
Virus Res. 2016 Sep 2;223:181-9
pubmed: 27497620
Virology. 1997 Sep 1;235(2):377-85
pubmed: 9281518
J Virol. 1996 Dec;70(12):8865-71
pubmed: 8971015
Sci Rep. 2020 Jan 16;10(1):494
pubmed: 31949276
Viruses. 2019 Jul 02;11(7):
pubmed: 31269702
J Virol. 2020 Mar 17;94(7):
pubmed: 31969432
J Virol. 2018 Nov 12;92(23):
pubmed: 30185597
Philos Trans R Soc Lond B Biol Sci. 2009 Sep 27;364(1530):2683-96
pubmed: 19687038
Viruses. 2020 Aug 21;12(9):
pubmed: 32825617
Virology. 1997 Apr 14;230(2):252-64
pubmed: 9143281
J Biol Chem. 2020 Jan 3;295(1):1-12
pubmed: 31649031
Science. 2019 Nov 1;366(6465):640-644
pubmed: 31624094
Viruses. 2020 Jun 23;12(6):
pubmed: 32585808
Virus Res. 2016 Feb 2;213:165-171
pubmed: 26656424
Sci Rep. 2018 Feb 16;8(1):3154
pubmed: 29453406
J Virol. 2015 Aug;89(16):8556-66
pubmed: 26063424
Virus Res. 1996 Feb;40(2):161-7
pubmed: 8725112
J Virol. 2015 Feb;89(4):2324-32
pubmed: 25505073
Viruses. 2020 Jan 03;12(1):
pubmed: 31947814
Virology. 1992 Jul;189(1):368-73
pubmed: 1604821
J Virol. 2016 Dec 16;91(1):
pubmed: 27795430