Altered Degranulation and pH of Neutrophil Phagosomes Impacts Antimicrobial Efficiency in Cystic Fibrosis.
bacterial phagosome
cystic fibrosis
degranulation
ion channel potentiator therapy
neutrophils
pH
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
28
08
2020
accepted:
16
11
2020
entrez:
4
1
2021
pubmed:
5
1
2021
medline:
17
6
2021
Statut:
epublish
Résumé
Studies have endeavored to understand the cause for impaired antimicrobial killing by neutrophils of people with cystic fibrosis (PWCF). The aim of this study was to focus on the bacterial phagosome. Possible alterations in degranulation of cytoplasmic granules and changes in pH were assessed. Circulating neutrophils were purified from PWCF (n = 28), PWCF receiving ivacaftor therapy (n = 10), and healthy controls (n = 28). Degranulation was assessed by Western blot analysis and flow cytometry. The pH of phagosomes was determined by use of BCECF-AM-labelled
Identifiants
pubmed: 33391268
doi: 10.3389/fimmu.2020.600033
pmc: PMC7775508
doi:
Types de publication
Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
600033Informations de copyright
Copyright © 2020 Hayes, Murphy, Pohl, Browne, McQuillan, Saw, Foley, Gargoum, McElvaney, Hawkins, Gunaratnam, McElvaney and Reeves.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Nature. 2002 Mar 21;416(6878):291-7
pubmed: 11907569
J Leukoc Biol. 2020 Dec;108(6):1777-1785
pubmed: 32531843
JCI Insight. 2020 Aug 6;5(15):
pubmed: 32634128
J Immunol. 1996 Sep 15;157(6):2728-35
pubmed: 8805680
Proc Natl Acad Sci U S A. 2009 May 5;106(18):7642-7
pubmed: 19372380
Nat Med. 2007 Dec;13(12):1423-30
pubmed: 18059279
Biochimie. 2008 Feb;90(2):227-42
pubmed: 18021746
Free Radic Biol Med. 2010 Dec 15;49(12):1872-80
pubmed: 20870018
J Leukoc Biol. 2016 Dec;100(6):1413-1424
pubmed: 27406994
Science. 1989 Sep 8;245(4922):1066-73
pubmed: 2475911
Am J Physiol Lung Cell Mol Physiol. 2000 Jan;278(1):L33-41
pubmed: 10645888
Sci Transl Med. 2014 Jan 1;6(217):217ra1
pubmed: 24382893
PLoS One. 2011;6(7):e22212
pubmed: 21811577
Eur J Biochem. 1975 Aug 1;56(1):157-62
pubmed: 170101
J Immunol. 2014 Mar 1;192(5):2418-31
pubmed: 24477913
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
PLoS One. 2017 Mar 9;12(3):e0173257
pubmed: 28278205
J Clin Invest. 2010 Dec;120(12):4236-50
pubmed: 21060150
Am J Respir Crit Care Med. 1996 Nov;154(5):1229-56
pubmed: 8912731
J Clin Invest. 1989 Oct;84(4):1302-13
pubmed: 2507578
Biochemistry. 2006 Aug 29;45(34):10260-9
pubmed: 16922501
J Biol Chem. 2006 May 5;281(18):12277-88
pubmed: 16522634
J Innate Immun. 2010;2(3):260-6
pubmed: 20375556
Am J Respir Crit Care Med. 2019 Dec 1;200(11):1381-1391
pubmed: 31454256
Annu Rev Immunol. 1993;11:105-28
pubmed: 8476558
Biochemistry. 2001 Aug 28;40(34):10204-12
pubmed: 11513598
J Immunol. 2009 Jun 1;182(11):7254-63
pubmed: 19454722
J Immunol. 2014 Feb 15;192(4):1787-95
pubmed: 24446515
J Biol Chem. 2019 Sep 6;294(36):13502-13514
pubmed: 31341024
Infect Immun. 1996 Sep;64(9):3512-7
pubmed: 8751892
Free Radic Biol Med. 2012 Dec 15;53(12):2308-17
pubmed: 23089227
Pediatr Pulmonol. 2011 Mar;46(3):230-8
pubmed: 20963831
J Clin Invest. 2020 Apr 1;130(4):2041-2053
pubmed: 31961827
N Engl J Med. 2011 Nov 3;365(18):1663-72
pubmed: 22047557
Am J Respir Crit Care Med. 1995 Dec;152(6 Pt 1):1910-6
pubmed: 8520754
Proc Soc Exp Biol Med. 1977 Mar;154(3):418-22
pubmed: 15280
Cell Signal. 1992 Sep;4(5):501-9
pubmed: 1419488
Blood. 2014 Aug 14;124(7):999-1009
pubmed: 24934256
Am J Physiol Lung Cell Mol Physiol. 2009 Jun;296(6):L859-67
pubmed: 19329540
J Cyst Fibros. 2016 Mar;15(2):147-57
pubmed: 26454351
Chem Res Toxicol. 1997 Oct;10(10):1080-9
pubmed: 9348429
Cytometry. 2002 Jul 1;48(3):159-66
pubmed: 12116362
J Clin Invest. 1967 Sep;46(9):1422-32
pubmed: 6036538
N Engl J Med. 2013 May 23;368(21):1963-70
pubmed: 23692169
Semin Hematol. 1968 Jul;5(3):215-54
pubmed: 5662845
J Allergy Clin Immunol. 2006 Jan;117(1):204-11
pubmed: 16387607
Front Pharmacol. 2020 Jul 17;11:1098
pubmed: 32765284
J Leukoc Biol. 2010 May;87(5):933-42
pubmed: 20089668
J Pediatr. 2009 Nov;155(5):623-8.e1
pubmed: 19616787
N Engl J Med. 1999 Jan 7;340(1):23-30
pubmed: 9878641
Am J Respir Crit Care Med. 1995 Aug;152(2):629-33
pubmed: 7633718
J Immunol. 2009 Jul 1;183(1):543-51
pubmed: 19542465
Infect Immun. 1999 Aug;67(8):4264-7
pubmed: 10417202
Infect Immun. 1988 Mar;56(3):693-8
pubmed: 3125111
J Leukoc Biol. 2008 Jun;83(6):1345-53
pubmed: 18353929
PLoS One. 2015 Apr 17;10(4):e0125906
pubmed: 25885273
J Pediatr Pharmacol Ther. 2020;25(3):192-197
pubmed: 32265602
J Cell Biol. 2007 Jan 15;176(2):231-41
pubmed: 17210947
Cytometry A. 2014 Jul;85(7):611-20
pubmed: 24623386
N Engl J Med. 2010 Nov 18;363(21):1991-2003
pubmed: 21083385
J Immunol. 1988 Sep 15;141(6):2097-103
pubmed: 2844894
EBioMedicine. 2017 Sep;23:173-184
pubmed: 28835336
Nature. 1981 Apr 2;290(5805):406-9
pubmed: 7219526
Am J Respir Crit Care Med. 1994 Jul;150(1):207-13
pubmed: 7912987
J Biol Chem. 2007 Oct 26;282(43):31422-8
pubmed: 17724021
J Exp Med. 1962 Dec 1;116:827-34
pubmed: 13961191
Infect Immun. 1999 Apr;67(4):1828-36
pubmed: 10085024
J Neurosci Methods. 2009 May 15;179(2):173-8
pubmed: 19428524
J Cell Biol. 1980 Apr;85(1):42-59
pubmed: 7364874
J Clin Invest. 1990 Jul;86(1):300-8
pubmed: 2164045
Nat Cell Biol. 2006 Sep;8(9):933-44
pubmed: 16921366
Am J Physiol Lung Cell Mol Physiol. 2000 Jul;279(1):L66-74
pubmed: 10893204
Thorax. 2001 Jan;56(1):62-4
pubmed: 11120907
J Immunol. 2018 Oct 1;201(7):2016-2027
pubmed: 30120123
Eur Respir J. 2003 Sep;22(3):497-502
pubmed: 14516141
Stem Cells. 2009 Jan;27(1):59-67
pubmed: 18845766