Interleukin 10 Restores Lipopolysaccharide-Induced Alterations in Synaptic Plasticity Probed by Repetitive Magnetic Stimulation.


Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2020
Historique:
received: 06 10 2020
accepted: 16 11 2020
entrez: 4 1 2021
pubmed: 5 1 2021
medline: 3 7 2021
Statut: epublish

Résumé

Systemic inflammation is associated with alterations in complex brain functions such as learning and memory. However, diagnostic approaches to functionally assess and quantify inflammation-associated alterations in synaptic plasticity are not well-established. In previous work, we demonstrated that bacterial lipopolysaccharide (LPS)-induced systemic inflammation alters the ability of hippocampal neurons to express synaptic plasticity, i.e., the long-term potentiation (LTP) of excitatory neurotransmission. Here, we tested whether synaptic plasticity induced by repetitive magnetic stimulation (rMS), a non-invasive brain stimulation technique used in clinical practice, is affected by LPS-induced inflammation. Specifically, we explored brain tissue cultures to learn more about the direct effects of LPS on neural tissue, and we tested for the plasticity-restoring effects of the anti-inflammatory cytokine interleukin 10 (IL10). As shown previously, 10 Hz repetitive magnetic stimulation (rMS) of organotypic entorhino-hippocampal tissue cultures induced a robust increase in excitatory neurotransmission onto CA1 pyramidal neurons. Furthermore, LPS-treated tissue cultures did not express rMS-induced synaptic plasticity. Live-cell microscopy in tissue cultures prepared from a novel transgenic reporter mouse line [

Identifiants

pubmed: 33391287
doi: 10.3389/fimmu.2020.614509
pmc: PMC7772211
doi:

Substances chimiques

IL10 protein, mouse 0
Interleukin-1beta 0
Interleukin-6 0
Lipopolysaccharides 0
Tumor Necrosis Factor-alpha 0
interleukin-6, mouse 0
Interleukin-10 130068-27-8
Interferon-gamma 82115-62-6

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

614509

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS109498
Pays : United States

Informations de copyright

Copyright © 2020 Lenz, Eichler, Kruse, Strehl, Rodriguez-Rozada, Goren, Yogev, Frank, Waisman, Deller, Jung, Maggio and Vlachos.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Maximilian Lenz (M)

Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Amelie Eichler (A)

Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Pia Kruse (P)

Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

Andreas Strehl (A)

Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Frankfurt, Germany.

Silvia Rodriguez-Rozada (S)

Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Frankfurt, Germany.

Itamar Goren (I)

Pharmazentrum Frankfurt/ZAFES, Goethe-University Frankfurt, Frankfurt, Germany.

Nir Yogev (N)

Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
Department of Dermatology, Faculty of Medicine, University of Cologne, Cologne, Germany.

Stefan Frank (S)

Pharmazentrum Frankfurt/ZAFES, Goethe-University Frankfurt, Frankfurt, Germany.

Ari Waisman (A)

Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.

Thomas Deller (T)

Institute of Clinical Neuroanatomy, Neuroscience Center, Goethe-University Frankfurt, Frankfurt, Germany.

Steffen Jung (S)

Department of Immunology, Weizmann Institute of Science, Rehovot, Israel.

Nicola Maggio (N)

Department of Neurology and Sagol Center for Neurosciences, Talpiot Medical Leadership Program, The Chaim Sheba Medical Center, Tel HaShomer, Israel.
Sackler Faculty of Medicine and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel.

Andreas Vlachos (A)

Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Center Brain Links Brain Tools, University of Freiburg, Freiburg, Germany.
Center for Basics in NeuroModulation (NeuroModulBasics), Faculty of Medicine, University of Freiburg, Freiburg, Germany.

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Classifications MeSH