Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus.
Angiotensin-Converting Enzyme 2
/ chemistry
COVID-19
/ prevention & control
Caco-2 Cells
/ virology
Cell Line
/ virology
Extracellular Vesicles
/ metabolism
Humans
Lentivirus
Receptors, Virus
/ metabolism
SARS-CoV-2
Serine Endopeptidases
/ metabolism
Spike Glycoprotein, Coronavirus
Virus Internalization
ACE2
EV therapy
SARS‐CoV‐2
TMPRSS2
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
23
07
2020
revised:
16
11
2020
accepted:
04
12
2020
entrez:
4
1
2021
pubmed:
5
1
2021
medline:
5
1
2021
Statut:
ppublish
Résumé
SARS-CoV-2 entry is mediated by binding of the spike protein (S) to the surface receptor ACE2 and subsequent priming by host TMPRSS2 allowing membrane fusion. Here, we produced extracellular vesicles (EVs) exposing ACE2 and demonstrate that ACE2-EVs are efficient decoys for SARS-CoV-2 S protein-containing lentivirus. Reduction of infectivity positively correlates with the level of ACE2, is much more efficient than with soluble ACE2 and further enhanced by the inclusion of TMPRSS2.
Identifiants
pubmed: 33391636
doi: 10.1002/jev2.12050
pii: JEV212050
pmc: PMC7769856
doi:
Substances chimiques
Receptors, Virus
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
ACE2 protein, human
EC 3.4.17.23
Angiotensin-Converting Enzyme 2
EC 3.4.17.23
Serine Endopeptidases
EC 3.4.21.-
TMPRSS2 protein, human
EC 3.4.21.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
e12050Informations de copyright
© 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
Références
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Nat Methods. 2017 Feb 28;14(3):228-232
pubmed: 28245209
PLoS One. 2015 Sep 17;10(9):e0138380
pubmed: 26379044
Cancer Res. 2001 Feb 15;61(4):1686-92
pubmed: 11245484
Cytotherapy. 2020 Sep;22(9):482-485
pubmed: 32425691
Science. 2016 Apr 15;352(6283):359-62
pubmed: 27013426
J Virol. 1995 Jun;69(6):3824-30
pubmed: 7745730
Clin Sci (Lond). 2020 Jun 26;134(12):1301-1304
pubmed: 32542396
J Exp Med. 2017 Jun 5;214(6):1769-1785
pubmed: 28484079
J Virol. 2004 Oct;78(19):10628-35
pubmed: 15367630
J Virol. 2014 Jan;88(2):1293-307
pubmed: 24227843
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Nature. 2003 Nov 27;426(6965):450-4
pubmed: 14647384
J Extracell Vesicles. 2019 Jul 03;8(1):1628592
pubmed: 31303981
Cell. 2020 May 28;181(5):1016-1035.e19
pubmed: 32413319
PLoS One. 2014 Nov 25;9(11):e113691
pubmed: 25423108
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7988-93
pubmed: 15897467
Nat Cell Biol. 2019 Jan;21(1):9-17
pubmed: 30602770
Nature. 2020 Mar;579(7798):260-264
pubmed: 32132711
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Sci Transl Med. 2019 May 15;11(492):
pubmed: 31092696
Am J Physiol Lung Cell Mol Physiol. 2009 Jul;297(1):L84-96
pubmed: 19411314
Cell. 2020 May 14;181(4):905-913.e7
pubmed: 32333836