Heterologous expression of Phanerochaete chrysosporium cellobiose dehydrogenase in Trichoderma reesei.
Catalytic constants
Cellobiose dehydrogenase
Cofactor occupancy
Glycosylation
Lignocellulose depolymerization
Phanerochaete chrysosporium
Redox potential
Trichoderma reesei
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
06 Jan 2021
06 Jan 2021
Historique:
received:
22
09
2020
accepted:
03
12
2020
entrez:
7
1
2021
pubmed:
8
1
2021
medline:
7
9
2021
Statut:
epublish
Résumé
Cellobiose dehydrogenase from Phanerochaete chrysosporium (PcCDH) is a key enzyme in lignocellulose depolymerization, biosensors and biofuel cells. For these applications, it should retain important molecular and catalytic properties when recombinantly expressed. While homologous expression is time-consuming and the prokaryote Escherichia coli is not suitable for expression of the two-domain flavocytochrome, the yeast Pichia pastoris is hyperglycosylating the enzyme. Fungal expression hosts like Aspergillus niger and Trichoderma reesei were successfully used to express CDH from the ascomycete Corynascus thermophilus. This study describes the expression of basidiomycetes PcCDH in T. reesei (PcCDH PcCDH Heterologous production of PcCDH
Sections du résumé
BACKGROUND
BACKGROUND
Cellobiose dehydrogenase from Phanerochaete chrysosporium (PcCDH) is a key enzyme in lignocellulose depolymerization, biosensors and biofuel cells. For these applications, it should retain important molecular and catalytic properties when recombinantly expressed. While homologous expression is time-consuming and the prokaryote Escherichia coli is not suitable for expression of the two-domain flavocytochrome, the yeast Pichia pastoris is hyperglycosylating the enzyme. Fungal expression hosts like Aspergillus niger and Trichoderma reesei were successfully used to express CDH from the ascomycete Corynascus thermophilus. This study describes the expression of basidiomycetes PcCDH in T. reesei (PcCDH
RESULTS
RESULTS
PcCDH
CONCLUSIONS
CONCLUSIONS
Heterologous production of PcCDH
Identifiants
pubmed: 33407462
doi: 10.1186/s12934-020-01492-0
pii: 10.1186/s12934-020-01492-0
pmc: PMC7789494
doi:
Substances chimiques
Recombinant Proteins
0
Cellobiose
16462-44-5
Carbohydrate Dehydrogenases
EC 1.1.-
cellobiose-quinone oxidoreductase
EC 1.1.99.18
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2Subventions
Organisme : European Research Council
ID : 726396
Pays : International
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