Algorithms to optimize multi-column chromatographic separations of proteins.
Column coupling
Monoclonal antibody
Multi-isocratic elution
On-column fractioning
Optimization
Protein analysis
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
25 Jan 2021
25 Jan 2021
Historique:
received:
28
09
2020
revised:
17
12
2020
accepted:
19
12
2020
pubmed:
11
1
2021
medline:
28
1
2021
entrez:
10
1
2021
Statut:
ppublish
Résumé
The goal of this work was to provide a technical solution for the automated optimization of multi-column systems for protein separation and fractionation. Both algorithm and a software that can be downloaded are provided. In this algorithm, the length and order of the individual column segments can be considered. Various solutions are provided by the algorithm, including i) to obtain uniform peak distribution, ii) to park the different species at the inlet of the individual column segments, and iii) to elute all species as a single peak. Two representative examples are presented, showing the possibility to obtain uniform selectivity between monoclonal antibody (mAb) sub-units, and the on-column fractioning of intact mAbs.
Identifiants
pubmed: 33422794
pii: S0021-9673(20)31112-2
doi: 10.1016/j.chroma.2020.461838
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
461838Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: