Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells.
Biomarkers
Cell Differentiation
Coculture Techniques
Culture Media, Conditioned
Cytotoxicity, Immunologic
Female
Humans
Immunophenotyping
Leukocytes, Mononuclear
/ immunology
Lymphocyte Count
Middle Aged
Neoplastic Stem Cells
/ immunology
Spheroids, Cellular
Stomach Neoplasms
/ etiology
T-Lymphocytes, Regulatory
/ immunology
Th17 Cells
/ immunology
Tumor Cells, Cultured
Journal
Journal of immunology research
ISSN: 2314-7156
Titre abrégé: J Immunol Res
Pays: Egypt
ID NLM: 101627166
Informations de publication
Date de publication:
2020
2020
Historique:
received:
05
06
2020
revised:
05
12
2020
accepted:
14
12
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
15
9
2021
Statut:
epublish
Résumé
Gastrosphere, an enriched cellular population with stem-like properties believed to be responsible for an escape from immune-mediated destruction. Th17 and Treg cells play a major role in gastric cancer; however, their interaction with gastrospheres remained elusive. Peripheral blood mononuclear cells were isolated from healthy donors and were cultured with conditioned media of MKN-45 (parental) cells as well as gastrospheres' conditioned media in the context of mixed lymphocyte reaction and in the presence of anti-CD3/CD28 beads. The proliferation was evaluated using CFSE staining; the percentages of CD4 The results revealed a decreased expansion of PBMCs postexposure to gastrospheres' conditioned medium which was concomitant with an increased percentage of Th17 and an enhanced Th17 to Treg ratio. The conditioned media of gastrospheres enhanced the secretion of IL-10 and IL-17 and decreased TGF- Our study showed that the condition media of gastrospheres can potentially induce Th17 with increasing in their cytotoxic effect. Based on our knowledge, the present study is the first study that emphasizes the role of gastrospheres in the induction of antitumor Th17 cells. However, it should be confirmed with complementary studies
Sections du résumé
BACKGROUND
BACKGROUND
Gastrosphere, an enriched cellular population with stem-like properties believed to be responsible for an escape from immune-mediated destruction. Th17 and Treg cells play a major role in gastric cancer; however, their interaction with gastrospheres remained elusive.
METHOD
METHODS
Peripheral blood mononuclear cells were isolated from healthy donors and were cultured with conditioned media of MKN-45 (parental) cells as well as gastrospheres' conditioned media in the context of mixed lymphocyte reaction and in the presence of anti-CD3/CD28 beads. The proliferation was evaluated using CFSE staining; the percentages of CD4
RESULTS
RESULTS
The results revealed a decreased expansion of PBMCs postexposure to gastrospheres' conditioned medium which was concomitant with an increased percentage of Th17 and an enhanced Th17 to Treg ratio. The conditioned media of gastrospheres enhanced the secretion of IL-10 and IL-17 and decreased TGF-
CONCLUSION
CONCLUSIONS
Our study showed that the condition media of gastrospheres can potentially induce Th17 with increasing in their cytotoxic effect. Based on our knowledge, the present study is the first study that emphasizes the role of gastrospheres in the induction of antitumor Th17 cells. However, it should be confirmed with complementary studies
Identifiants
pubmed: 33426090
doi: 10.1155/2020/6261814
pmc: PMC7775146
doi:
Substances chimiques
Biomarkers
0
Culture Media, Conditioned
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6261814Informations de copyright
Copyright © 2020 Alaleh Rezalotfi et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflict of interests.
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