Multistage and transmission-blocking targeted antimalarials discovered from the open-source MMV Pandemic Response Box.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
11 01 2021
Historique:
received: 22 05 2020
accepted: 10 12 2020
entrez: 12 1 2021
pubmed: 13 1 2021
medline: 22 1 2021
Statut: epublish

Résumé

Chemical matter is needed to target the divergent biology associated with the different life cycle stages of Plasmodium. Here, we report the parallel de novo screening of the Medicines for Malaria Venture (MMV) Pandemic Response Box against Plasmodium asexual and liver stage parasites, stage IV/V gametocytes, gametes, oocysts and as endectocides. Unique chemotypes were identified with both multistage activity or stage-specific activity, including structurally diverse gametocyte-targeted compounds with potent transmission-blocking activity, such as the JmjC inhibitor ML324 and the antitubercular clinical candidate SQ109. Mechanistic investigations prove that ML324 prevents histone demethylation, resulting in aberrant gene expression and death in gametocytes. Moreover, the selection of parasites resistant to SQ109 implicates the druggable V-type H

Identifiants

pubmed: 33431834
doi: 10.1038/s41467-020-20629-8
pii: 10.1038/s41467-020-20629-8
pmc: PMC7801607
doi:

Substances chimiques

Antimalarials 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

269

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI152533
Pays : United States

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Auteurs

Janette Reader (J)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa.

Mariëtte E van der Watt (ME)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa.

Dale Taylor (D)

Drug Discovery and Development Centre (H3D), University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.

Claire Le Manach (C)

Drug Discovery and Development Centre (H3D), University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.

Nimisha Mittal (N)

Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093-076, USA.

Sabine Ottilie (S)

Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093-076, USA.

Anjo Theron (A)

Next Generation Health, Council for Scientific and Industrial Research, Pretoria, 0001, South Africa.

Phanankosi Moyo (P)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa.

Erica Erlank (E)

Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, and Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, 2193, South Africa.

Luisa Nardini (L)

Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, and Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, 2193, South Africa.

Nelius Venter (N)

Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, and Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, 2193, South Africa.

Sonja Lauterbach (S)

Department of Molecular Medicine and Hematology, Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 2193, South Africa.

Belinda Bezuidenhout (B)

Department of Molecular Medicine and Hematology, Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 2193, South Africa.

Andre Horatscheck (A)

Drug Discovery and Development Centre (H3D), University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.

Ashleigh van Heerden (A)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa.

Natalie J Spillman (NJ)

Division of Infectious Diseases, Department of Medicine, Washington University, St. Louis, MO, 63110, USA.

Anne N Cowell (AN)

Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093-076, USA.

Jessica Connacher (J)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa.

Daniel Opperman (D)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa.

Lindsey M Orchard (LM)

Department of Biochemistry & Molecular Biology and the Huck Centre for Malaria Research, Pennsylvania State University, University Park, PA, 16802, USA.

Manuel Llinás (M)

Department of Biochemistry & Molecular Biology and the Huck Centre for Malaria Research, Pennsylvania State University, University Park, PA, 16802, USA.
Department of Chemistry, Pennsylvania State University, University Park, PA, 16802, USA.

Eva S Istvan (ES)

Division of Infectious Diseases, Department of Medicine, Washington University, St. Louis, MO, 63110, USA.

Daniel E Goldberg (DE)

Division of Infectious Diseases, Department of Medicine, Washington University, St. Louis, MO, 63110, USA.

Grant A Boyle (GA)

Drug Discovery and Development Centre (H3D), University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.

David Calvo (D)

Global Health Incubator Unit, GlaxoSmithKline (GSK), Severo Ochoa, 2, 28760, Tres Cantos, Madrid, Spain.

Dalu Mancama (D)

Next Generation Health, Council for Scientific and Industrial Research, Pretoria, 0001, South Africa.

Theresa L Coetzer (TL)

Department of Molecular Medicine and Hematology, Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 2193, South Africa.

Elizabeth A Winzeler (EA)

Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093-076, USA.

James Duffy (J)

Medicines for Malaria Venture, International Center Cointrin, Route de Pré-Bois 20, 1215, Geneva, Switzerland.

Lizette L Koekemoer (LL)

Wits Research Institute for Malaria, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, and Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg, 2193, South Africa.

Gregory Basarab (G)

Drug Discovery and Development Centre (H3D), University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.

Kelly Chibale (K)

Drug Discovery and Development Centre (H3D), University of Cape Town, Rondebosch, Cape Town, 7701, South Africa.
South African Medical Research Council, Drug Discovery and Development Research Unit, Department of Chemistry and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Rondebosch, 7701, South Africa.

Lyn-Marié Birkholtz (LM)

Department of Biochemistry, Genetics and Microbiology, Institute for Sustainable Malaria Control, University of Pretoria, Hatfield, Pretoria, 0028, South Africa. lbirkholtz@up.ac.za.

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