Characterization of the ability of a, second-generation SST-DA chimeric molecule, TBR-065, to suppress GH secretion from human GH-secreting adenoma cells.


Journal

Pituitary
ISSN: 1573-7403
Titre abrégé: Pituitary
Pays: United States
ID NLM: 9814578

Informations de publication

Date de publication:
Jun 2021
Historique:
accepted: 17 11 2020
pubmed: 13 1 2021
medline: 15 12 2021
entrez: 12 1 2021
Statut: ppublish

Résumé

Somatostatin (SST) and dopamine (DA) inhibit growth hormone (GH) secretion and proliferation of GH-secreting pituitary adenomas (GHomas) through binding to SSTR2 and D2R receptors. Chimeric SST-DA compounds (Dopastatins) display increased potency in inhibiting GH secretion, as compared with individual SST or DA analogs (alone or combined). To assess the efficacy of a second-generation dopastatin, TBR-065, in suppressing GH secretion from human GH- and GH/prolactin(PRL)-omas. We compared the ability of TBR-065 to inhibit GH secretion from primary cultures of human GH- or GH/PRLoma cells to that of the first generation dopastatin, TBR-760 (formerly BIM-23A760), octreotide (OCT) and cabergoline (CAB), the later either alone or combined. We investigated whether there was any impact of BIM-133, the metabolite of TBR-065, on the ability of TBR-065 to inhibit GH in these cultures. 17 GH- and GH/PRLomas were included in this study. Inhibition of GH secretion by TBR-065, TBR-760, OCT and CAB (0.1 pM to 0.1 µM) was assessed over a period of 8 h. All tumors expressed SSTR2 and D2R mRNAs. GH suppression was higher with TBR-065 as compared with TBR-760 (E TBR-065 has significantly improved efficacy in suppressing GH secretion as compared to current available therapies and may represent a new promising option for the treatment of acromegaly.

Identifiants

pubmed: 33433890
doi: 10.1007/s11102-020-01113-4
pii: 10.1007/s11102-020-01113-4
doi:

Substances chimiques

Receptors, Dopamine D2 0
Receptors, Somatostatin 0
Human Growth Hormone 12629-01-5
Somatostatin 51110-01-1
Cabergoline LL60K9J05T
Octreotide RWM8CCW8GP
Dopamine VTD58H1Z2X

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

351-358

Références

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Auteurs

Thomas Cuny (T)

Service d'Endocrinologie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Conception, 147 Boulevard Baille, 13005, Marseille, France. thomas.cuny@ap-hm.fr.

Thomas Graillon (T)

Service de Neurochirurgie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Timone, Marseille, France.

Célines Defilles (C)

Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Marseille, France.

Rakesh Datta (R)

Ipsen Bioscience (Formers Employees), Cambridge, MA, USA.

Shengwen Zhang (S)

Ipsen Bioscience (Formers Employees), Cambridge, MA, USA.

Dominique Figarella-Branger (D)

Laboratoire d'Anatomopathologie, Aix Marseille University, APHM, Hôpital de la Timone, Marseille, France.

Henry Dufour (H)

Service de Neurochirurgie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Timone, Marseille, France.

Grégory Mougel (G)

Laboratoire de Biologie Moléculaire et Biochimie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Conception, Marseille, France.

Thierry Brue (T)

Service d'Endocrinologie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Conception, 147 Boulevard Baille, 13005, Marseille, France.

Tanya Landsman (T)

Ipsen Bioscience (Formers Employees), Cambridge, MA, USA.

Heather A Halem (HA)

Tiburio Therapeutics, Cambridge, MA, USA.

Michael D Culler (MD)

Tiburio Therapeutics, Cambridge, MA, USA.

Anne Barlier (A)

Laboratoire de Biologie Moléculaire et Biochimie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Conception, Marseille, France.

Alexandru Saveanu (A)

Laboratoire de Biologie Moléculaire et Biochimie, Aix Marseille University, APHM, Marseille Medical Genetics, Inserm U1251, Hôpital de la Conception, Marseille, France.

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