In-Depth Characterization of the Staphylococcus aureus Phosphoproteome Reveals New Targets of Stk1.

Bacillus subtilis / metabolism Bacterial Proteins / genetics Phosphopeptides / genetics Phosphoproteins / genetics Phosphorylation Protein Kinases / metabolism Proteome Staphylococcus aureus / genetics
Microbiology bacteria mass spectrometry pathogens phosphatases phosphorylation serine/threonine kinases substrate identification

Journal

Molecular & cellular proteomics : MCP
ISSN: 1535-9484
Titre abrégé: Mol Cell Proteomics
Pays: United States
ID NLM: 101125647

Informations de publication

Date de publication:
2021
Historique:
received: 17 07 2020
revised: 02 12 2020
accepted: 10 12 2020
pubmed: 15 1 2021
medline: 15 3 2022
entrez: 14 1 2021
Statut: ppublish

Résumé

Staphylococcus aureus is a major cause of infections worldwide, and infection results in a variety of diseases. As of no surprise, protein phosphorylation is an important game player in signaling cascades and has been shown to be involved in S. aureus virulence. Albeit long neglected, eukaryotic-type serine/threonine kinases in S. aureus have been implicated in this complex signaling cascades. Due to the substoichiometric nature of protein phosphorylation and a lack of suitable analysis tools, the knowledge of these cascades is, however, to date, still limited. Here, were apply an optimized protocol for efficient phosphopeptide enrichment via Fe

Identifiants

DOI: 10.1074/mcp.RA120.002232 PMID: 33444734 PMC: PMC7950182
pubmed: 33444734
pii: S1535-9476(20)35148-3
doi: 10.1074/mcp.RA120.002232
pmc: PMC7950182
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Phosphopeptides 0
Phosphoproteins 0
Proteome 0
Protein Kinases EC 2.7.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

100034

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest The authors declare no competing financial interest.

Auteurs

Nadine Prust (N)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Netherlands Proteomics Center, Utrecht, the Netherlands.

Saar van der Laarse (S)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Netherlands Proteomics Center, Utrecht, the Netherlands.

Henk W P van den Toorn (HWP)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Netherlands Proteomics Center, Utrecht, the Netherlands.

Nina M van Sorge (NM)

Medical Microbiology, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands; Department of Medical Microbiology and Infection Prevention and Netherlands Reference Laboratory for Bacterial Meningitis, Amsterdam University Medical Center, University of Amsterdam, Amsterdam, the Netherlands.

Simone Lemeer (S)

Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands; Netherlands Proteomics Center, Utrecht, the Netherlands. Electronic address: s.m.lemeer@uu.nl.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › In-Depth Characterization of the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › In-Depth Characterization of the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Phosphopeptides › In-Depth Characterization of the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › In-Depth Characterization of the...
questionsmedicales.fr › Métabolisme › Phosphorylation › In-Depth Characterization of the...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Phosphotransferases (Alcohol Group Acceptor) › Protein kinases › In-Depth Characterization of the...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › In-Depth Characterization of the...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › In-Depth Characterization of the...