Identification of distinct pH- and zeaxanthin-dependent quenching in LHCSR3 from
chlamydomonas reinhardtii
fluorescence spectroscopy
molecular biophysics
non-photochemical quenching
photosynthetic light harvesting
plant biology
structural biology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
15 01 2021
15 01 2021
Historique:
received:
24
06
2020
accepted:
14
01
2021
pubmed:
16
1
2021
medline:
4
2
2022
entrez:
15
1
2021
Statut:
epublish
Résumé
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as a quenching site. Using a combined in vivo and in vitro approach, we investigated quenching within LHCSR3 from Green plants and algae rely on sunlight to transform light energy into chemical energy in a process known as photosynthesis. However, too much light can damage plants. Green plants prevent this by converting the extra absorbed light into heat. Both the absorption and the dissipation of sunlight into heat occur within so called light harvesting complexes. These are protein structures that contain pigments such as chlorophyll and carotenoids. The process of photoprotection starts when the excess of absorbed light generates protons (elementary particles with a positive charge) faster than they can be used. This causes a change in the pH (a measure of the concentration of protons in a solution), which in turn, modifies the shape of proteins and the chemical identity of the carotenoids. However, it is still unclear what the exact mechanisms are. To clarify this, Troiano, Perozeni et al. engineered the light harvesting complex LHCSR3 of the green algae
Autres résumés
Type: plain-language-summary
(eng)
Green plants and algae rely on sunlight to transform light energy into chemical energy in a process known as photosynthesis. However, too much light can damage plants. Green plants prevent this by converting the extra absorbed light into heat. Both the absorption and the dissipation of sunlight into heat occur within so called light harvesting complexes. These are protein structures that contain pigments such as chlorophyll and carotenoids. The process of photoprotection starts when the excess of absorbed light generates protons (elementary particles with a positive charge) faster than they can be used. This causes a change in the pH (a measure of the concentration of protons in a solution), which in turn, modifies the shape of proteins and the chemical identity of the carotenoids. However, it is still unclear what the exact mechanisms are. To clarify this, Troiano, Perozeni et al. engineered the light harvesting complex LHCSR3 of the green algae
Identifiants
pubmed: 33448262
doi: 10.7554/eLife.60383
pii: 60383
pmc: PMC7864637
doi:
pii:
Substances chimiques
Algal Proteins
0
Zeaxanthins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Troiano et al.
Déclaration de conflit d'intérêts
JT, FP, RM, LZ, KB, EJ, SC, MB, GS No competing interests declared
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