Duplex SYBR Green I-based real-time PCR assay for the rapid detection of canine kobuvirus and canine astrovirus.

Animals Benzothiazoles Diamines Dogs Kobuvirus / genetics Organic Chemicals Quinolines Real-Time Polymerase Chain Reaction Reproducibility of Results Sensitivity and Specificity
Canine astrovirus Canine kobuvirus Duplex real-time PCR

Journal

Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839

Informations de publication

Date de publication:
04 2021
Historique:
received: 09 08 2020
revised: 11 01 2021
accepted: 11 01 2021
pubmed: 17 1 2021
medline: 28 10 2021
entrez: 16 1 2021
Statut: ppublish

Résumé

A duplex SYBR Green I-based real-time PCR assay was established for the simultaneous detection of canine kobuvirus (CaKoV) and canine astrovirus (CaAstV). This assay can easily distinguish the two viruses according to their different melting temperatures (Tm) of 80 °C for CaKoV and 86.5 °C for CaAstV; other canine enteroviruses used as controls showed no specific melting peaks. The detection limit of this assay was determined to be 10

Identifiants

DOI: 10.1016/j.jviromet.2021.114066 PMID: 33453300
pubmed: 33453300
pii: S0166-0934(21)00005-7
doi: 10.1016/j.jviromet.2021.114066
pii:
doi:

Substances chimiques

Benzothiazoles 0
Diamines 0
Organic Chemicals 0
Quinolines 0
SYBR Green I 163795-75-3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

114066

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Yong Wang (Y)

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China.

Yeqiu Li (Y)

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China.

Yongqiu Cui (Y)

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China.

Shudong Jiang (S)

Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, PR China.

Hua Liu (H)

Anhui Provincial Center for Animal Disease Control and Prevention, Hefei, 230000, PR China.

Jing Wang (J)

Animal Husbandry Base Teaching and Research Section, College of Animal Science and Technology, Hebei North University, Zhangjiakou, 075131, PR China. Electronic address: wangjing@hebeinu.com.cn.

Yongdong Li (Y)

Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, PR China. Electronic address: liyd0551@126.com.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Duplex SYBR Green I-based real-time PCR assay...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Benzothiazoles › Duplex SYBR Green I-based real-time PCR assay...
questionsmedicales.fr › Composés chimiques organiques › Amines › Polyamines › Diamines › Duplex SYBR Green I-based real-time PCR assay...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Carnivora › Pinnipedia › Canidae › Chiens › Duplex SYBR Green I-based real-time PCR assay...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Picornaviridae › Kobuvirus › Duplex SYBR Green I-based real-time PCR assay...
questionsmedicales.fr › Composés chimiques organiques › Duplex SYBR Green I-based real-time PCR assay...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Quinoléines › Duplex SYBR Green I-based real-time PCR assay...
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