A novel pathogenicity determinant hijacks maize catalase 1 to enhance viral multiplication and infection.
Zea mays
catalase
maize chlorotic mottle virus (MCMV)
pathogenesis-related (PR) protein
salicylic acid (SA)-mediated defence
systemic necrosis
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
17
11
2020
accepted:
07
01
2021
pubmed:
19
1
2021
medline:
15
5
2021
entrez:
18
1
2021
Statut:
ppublish
Résumé
Pathogens have evolved various strategies to overcome host immunity for successful infection. Maize chlorotic mottle virus (MCMV) can cause lethal necrosis in maize (Zea mays) when it coinfects with a virus in the Potyviridae family. However, the MCMV pathogenicity determinant remains largely unknown. Here we show that the P31 protein of MCMV is important for viral accumulation and essential for symptom development. Ectopic expression of P31 using foxtail mosaic virus or potato virus X induced necrosis in systemically infected maize or Nicotiana benthamiana leaves. Maize catalases (CATs) were shown to interact with P31 in yeast and in planta. P31 accumulation was elevated through its interaction with ZmCAT1. P31 attenuated the expression of salicylic acid (SA)-responsive pathogenesis-related (PR) genes by inhibiting catalase activity during MCMV infection. In addition, silencing of ZmCATs using a brome mosaic virus-based gene silencing vector facilitated MCMV RNA and coat protein accumulation. This study reveals an important role for MCMV P31 in counteracting host defence and inducing systemic chlorosis and necrosis. Our results have implications for understanding the mechanisms in defence and counter-defence during infection of plants by various pathogens.
Substances chimiques
Catalase
EC 1.11.1.6
Salicylic Acid
O414PZ4LPZ
Banques de données
RefSeq
['NM_001254879.2', 'NM_001111840.2', 'NM_001363892.1', 'NM_001367877.1', 'EU970195.1']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1126-1141Informations de copyright
© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation.
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