Using galactitol dehydrogenase coupled with water-forming NADH oxidase for efficient enzymatic synthesis of L-tagatose.
Bi-enzyme
Bio-catalysis
Galactitol dehydrogenase
L-tagatose
NAD(+) regeneration
Water-forming NADH oxidase
Journal
New biotechnology
ISSN: 1876-4347
Titre abrégé: N Biotechnol
Pays: Netherlands
ID NLM: 101465345
Informations de publication
Date de publication:
25 May 2021
25 May 2021
Historique:
received:
27
04
2020
revised:
05
01
2021
accepted:
11
01
2021
pubmed:
19
1
2021
medline:
5
10
2021
entrez:
18
1
2021
Statut:
ppublish
Résumé
L-Tagatose, a promising building block in the production of many value-added chemicals, is generally produced by chemical routes with a low yield, which may not meet the increasing demands. Synthesis of l-tagatose by enzymatic oxidation of d-galactitol has not been applied on an industrial scale because of the high cofactor costs and the lack of efficient cofactor regeneration methods. In this work, an efficient and environmentally friendly enzymatic method containing a galactitol dehydrogenase for d-galactitol oxidation and a water-forming NADH oxidase for regeneration of NAD
Identifiants
pubmed: 33460816
pii: S1871-6784(21)00005-4
doi: 10.1016/j.nbt.2021.01.003
pii:
doi:
Substances chimiques
Hexoses
0
Multienzyme Complexes
0
Sugar Alcohol Dehydrogenases
EC 1.1.-
galactitol 2-dehydrogenase
EC 1.1.1.16
NADH oxidase
EC 1.6.-
NADH, NADPH Oxidoreductases
EC 1.6.-
tagatose
T7A20Y888Y
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18-25Informations de copyright
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