Gene expression comparison between primary estrogen receptor-positive and triple-negative breast cancer with paired axillary lymph node metastasis.
estrogen receptor-positive breast cancer
gene expression
lymph node metastasis
triple-negative breast cancer
Journal
The breast journal
ISSN: 1524-4741
Titre abrégé: Breast J
Pays: United States
ID NLM: 9505539
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
06
11
2020
received:
22
09
2020
accepted:
10
11
2020
pubmed:
20
1
2021
medline:
9
7
2021
entrez:
19
1
2021
Statut:
ppublish
Résumé
The aim of this study is to characterize and compare changes in gene expression patterns of paired axillary lymph node (ALN) metastases from estrogen receptor (ER)-positive and triple-negative (TNBC) primary breast cancer (PBC). Patients with stage 2-3 PBC with macrometastasis to an ALN were selected. Gene expression of 2567 cancer-associated genes was analyzed with the HTG EdgeSeq system coupled with the Illumina Next Generation Sequencing (NGS) platform. Changes in gene expression between ER/PR-positive, HER2-negative PBC, and their paired ALN metastases were compared with TNBC and their paired ALN metastases. Fourteen pairs of ER-positive and paired ALN metastasis were analyzed. Compared with the PBC, ALN metastasis had 673 significant differentially expressed genes, including 348 upregulated genes and 325 downregulated genes. Seventeen pairs of TNBC and paired ALN metastasis were analyzed. ALN metastasis had 257 significant differentially expressed genes, including 123 upregulated genes and 134 downregulated genes. When gene expression of the ALN for ER-positive PBC was compared to that of TNBC, 97 genes were upregulated in both, and 115 genes were similarly downregulated. Common upregulated genes were associated with cell death, necrosis, and homeostasis. Common downregulated genes were those of migration, degradation of extracellular matrix, and invasion. Although ER-positive PBC and TNBC have a distinct gene expression profiles and distinct changes from PBC to ALN metastases, a significant number of genes are similarly up- or downregulated. Understanding the role of these common genomic changes may provide clues to understanding the metastatic process itself.
Identifiants
pubmed: 33464691
doi: 10.1111/tbj.14119
pmc: PMC8137535
mid: NIHMS1666712
doi:
Substances chimiques
Receptors, Estrogen
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
432-440Subventions
Organisme : Community Outreach and Engagement Developmental Fund Award
Organisme : U.S. Department of Defense
ID : W81XWH-18-1-0067
Organisme : Fashion Footwear Association of New York
Organisme : NCI NIH HHS
ID : R01 CA151610
Pays : United States
Organisme : Margie and Robert E. Petersen Foundation
Organisme : Samuel Oschin Cancer Institute Discovery Fund Award
Organisme : NIH Clinical Center
ID : 2R01CA151610
Organisme : Linda and Jim Lippman
Informations de copyright
© 2021 Wiley Periodicals LLC.
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