Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
15
05
2020
accepted:
04
12
2020
revised:
29
01
2021
pubmed:
20
1
2021
medline:
23
4
2021
entrez:
19
1
2021
Statut:
epublish
Résumé
Hybridization has resulted in the origin and variation in extant species, and hybrids continue to arise despite pre- and post-zygotic barriers that limit their formation and evolutionary success. One important system that maintains species boundaries in prokaryotes and eukaryotes is the mismatch repair pathway, which blocks recombination between divergent DNA sequences. Previous studies illuminated the role of the mismatch repair component Msh2 in blocking genetic recombination between divergent DNA during meiosis. Loss of Msh2 results in increased interspecific genetic recombination in bacterial and yeast models, and increased viability of progeny derived from yeast hybrid crosses. Hybrid isolates of two pathogenic fungal Cryptococcus species, Cryptococcus neoformans and Cryptococcus deneoformans, are isolated regularly from both clinical and environmental sources. In the present study, we sought to determine if loss of Msh2 would relax the species boundary between C. neoformans and C. deneoformans. We found that crosses between these two species in which both parents lack Msh2 produced hybrid progeny with increased viability and high levels of aneuploidy. Whole-genome sequencing revealed few instances of recombination among hybrid progeny and did not identify increased levels of recombination in progeny derived from parents lacking Msh2. Several hybrid progeny produced structures associated with sexual reproduction when incubated alone on nutrient-rich medium in light, a novel phenotype in Cryptococcus. These findings represent a unique, unexpected case where rendering the mismatch repair system defective did not result in increased meiotic recombination across a species boundary. This suggests that alternative pathways or other mismatch repair components limit meiotic recombination between homeologous DNA and enforce species boundaries in the basidiomycete Cryptococcus species.
Identifiants
pubmed: 33465111
doi: 10.1371/journal.pgen.1008871
pii: PGENETICS-D-20-00781
pmc: PMC7846113
doi:
Substances chimiques
MutS Homolog 2 Protein
EC 3.6.1.3
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008871Subventions
Organisme : NIAID NIH HHS
ID : R37 AI039115
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007184
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI039115
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI100272
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI050113
Pays : United States
Organisme : NIAID NIH HHS
ID : F31 AI143136
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI039115
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI100272
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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