Combining Colistin and Fluconazole Synergistically Increases Fungal Membrane Permeability and Antifungal Cidality.

antifungal azole colistin ergosterol synthesis membrane permeabilization yeast

Journal

ACS infectious diseases
ISSN: 2373-8227
Titre abrégé: ACS Infect Dis
Pays: United States
ID NLM: 101654580

Informations de publication

Date de publication:
12 02 2021
Historique:
pubmed: 21 1 2021
medline: 24 6 2021
entrez: 20 1 2021
Statut: ppublish

Résumé

The increasing emergence of drug-resistant fungal pathogens, together with the limited number of available antifungal drugs, presents serious clinical challenges to treating systemic, life-threatening infections. Repurposing existing drugs to augment the antifungal activity of well-tolerated antifungals is a promising antifungal strategy with the potential to be implemented rapidly. Here, we explored the mechanism by which colistin, a positively charged lipopeptide antibiotic, enhances the antifungal activity of fluconazole, the most widely used orally available antifungal. In a range of susceptible and drug-resistant isolates and species, colistin was primarily effective at reducing fluconazole tolerance, a property of subpopulations of cells that grow slowly in the presence of a drug and may promote the emergence of persistent infections and resistance. Clinically relevant concentrations of colistin synergized with fluconazole, reducing fluconazole minimum inhibitory concentration 4-fold. Combining fluconazole and colistin also increased survival in a

Identifiants

pubmed: 33471513
doi: 10.1021/acsinfecdis.0c00721
pmc: PMC7887753
doi:

Substances chimiques

Antifungal Agents 0
Fluconazole 8VZV102JFY
Colistin Z67X93HJG1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

377-389

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Auteurs

Maayan Bibi (M)

Shmunis School of Biomedical and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 6997801, Israel.

Sarah Murphy (S)

Institute for Infection and Immunity, St George's University, Cranmer Terrace, London SW17 0RE, United Kingdom.

Raphael I Benhamou (RI)

School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Ramat Aviv 6997801, Israel.

Alex Rosenberg (A)

Shmunis School of Biomedical and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 6997801, Israel.

Adi Ulman (A)

Shmunis School of Biomedical and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 6997801, Israel.

Tihana Bicanic (T)

Institute for Infection and Immunity, St George's University, Cranmer Terrace, London SW17 0RE, United Kingdom.
Clinical Academic Group in Infection, St George's Hospital NHS Trust, London SW17 0QT, United Kingdom.

Micha Fridman (M)

School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Ramat Aviv 6997801, Israel.

Judith Berman (J)

Shmunis School of Biomedical and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 6997801, Israel.

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