Immunological features of circulating monocyte subsets in patients with squamous cell carcinoma of the head and neck.
Adult
Aged
Aged, 80 and over
B7-H1 Antigen
/ metabolism
Blood Circulation
Female
Flow Cytometry
HLA-G Antigens
Head and Neck Neoplasms
/ immunology
Humans
Immunophenotyping
Lipopolysaccharide Receptors
/ metabolism
Male
Middle Aged
Monocytes
/ immunology
Receptors, IgG
/ metabolism
Squamous Cell Carcinoma of Head and Neck
/ immunology
Up-Regulation
HLA-G
Intermediate monocytes
Monocyte subsets
PD-L1
Squamous cell carcinoma of the head and neck
Journal
Clinical immunology (Orlando, Fla.)
ISSN: 1521-7035
Titre abrégé: Clin Immunol
Pays: United States
ID NLM: 100883537
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
08
05
2020
revised:
23
11
2020
accepted:
08
01
2021
pubmed:
22
1
2021
medline:
12
6
2021
entrez:
21
1
2021
Statut:
ppublish
Résumé
Circulating monocytes are classified into three subsets according to their CD14 and CD16 expressions. Here we investigated all three subsets in patients with squamous cell carcinoma of the head and neck (SCCHN). Peripheral blood from 54 patients with SCCHN and 24 healthy donors (HDs) was tested for flowcytometry. Immunohistochemical staining of the primary tumor was performed. SCCHN cells were co-cultured with human monocytes in vitro. The level of intermediate monocytes was significantly lower in SCCHN than in HDs. The expression levels of HLA-G, PD-L1, and CD51 on intermediate monocytes was evidently greater in patients with SCCHN. In vitro co-culturing of SCCHN cells with monocytes revealed a significant increase in CD51 expression levels on monocytes. The decrease in expression levels of the maturation markers CX3CR1 and CD68 was significantly correlated to poor clinical outcomes. The level of intermediate monocytes was decreased in cancer patients in favor of immature and expressed immunosuppressive molecules.
Sections du résumé
BACKGROUND
Circulating monocytes are classified into three subsets according to their CD14 and CD16 expressions. Here we investigated all three subsets in patients with squamous cell carcinoma of the head and neck (SCCHN).
METHODS
Peripheral blood from 54 patients with SCCHN and 24 healthy donors (HDs) was tested for flowcytometry. Immunohistochemical staining of the primary tumor was performed. SCCHN cells were co-cultured with human monocytes in vitro.
RESULTS
The level of intermediate monocytes was significantly lower in SCCHN than in HDs. The expression levels of HLA-G, PD-L1, and CD51 on intermediate monocytes was evidently greater in patients with SCCHN. In vitro co-culturing of SCCHN cells with monocytes revealed a significant increase in CD51 expression levels on monocytes. The decrease in expression levels of the maturation markers CX3CR1 and CD68 was significantly correlated to poor clinical outcomes.
CONCLUSION
The level of intermediate monocytes was decreased in cancer patients in favor of immature and expressed immunosuppressive molecules.
Identifiants
pubmed: 33476742
pii: S1521-6616(21)00014-0
doi: 10.1016/j.clim.2021.108677
pii:
doi:
Substances chimiques
B7-H1 Antigen
0
HLA-G Antigens
0
Lipopolysaccharide Receptors
0
Receptors, IgG
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108677Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.