Chondrocytes from osteoarthritic cartilage of obese patients show altered adiponectin receptors expression and response to adiponectin.
Adiponectin
Cartilage
/ metabolism
Cartilage, Articular
/ pathology
Chondrocytes
/ metabolism
Diabetes Mellitus, Type 2
Female
Humans
Interleukin-6
/ metabolism
Male
Matrix Metalloproteinase 1
/ metabolism
Matrix Metalloproteinase 2
/ metabolism
Obesity
/ metabolism
Osteoarthritis
/ metabolism
Receptors, Adiponectin
/ genetics
Vascular Cell Adhesion Molecule-1
/ metabolism
BMI
OA
adipokines
adiponectin
arthritis
cartilage
obesity
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
27
11
2020
received:
02
09
2020
accepted:
19
01
2021
pubmed:
24
1
2021
medline:
19
4
2022
entrez:
23
1
2021
Statut:
ppublish
Résumé
Obesity and osteoarthritis (OA) are well-known comorbidities and their precise molecular interactions are still unidentified. Adiponectin, a major adipokine, known to have an anti-inflammatory effect in atherosclerosis or Type 2 Diabetes Mellitus (T2DM), has also been postulated to be pro-inflammatory in OA. This dual role of adiponectin is still not explained. The precise mechanism by which adiponectin affects cartilage and chondrocytes remains to be elucidated. In the present observational study chondrocytes from 30 patients with OA (18 females and 12 males) undergoing total knee replacement (TKR) were isolated. Expression of adiponectin receptors 1 and 2 (ADIPOR1 and ADIPOR2) was examined both at gene and protein levels in chondrocytes. The difference in adiponectin receptor expression between lean and obese patients with OA and the role of adiponectin in regulating pro-inflammatory genes (MCP-1, IL-6, and VCAM-1, MMP-1, MMP-2, and TIMP-1) has been investigated. We found that ADIPOR1 represented the most abundant adiponectin receptor in primary OA chondrocytes. ADIPOR1 and ADIPOR2 genes and ADIPOR1 protein were differently expressed in OA chondrocytes obtained from obese compared with lean patients with OA. Adiponectin induced gene expression of MCP-1, IL-6, and MMP-1 in all OA patients' chondrocytes. In contrast, VCAM-1 and MMP-2 were differently regulated by adiponectin depending on the patient's body mass index. This study suggests that adiponectin and ADIPOR1 may have important roles in the pathogenesis of cartilage degeneration in OA of obese subjects.
Substances chimiques
Adiponectin
0
Interleukin-6
0
Receptors, Adiponectin
0
Vascular Cell Adhesion Molecule-1
0
Matrix Metalloproteinase 2
EC 3.4.24.24
Matrix Metalloproteinase 1
EC 3.4.24.7
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
2333-2339Informations de copyright
© 2021 Orthopaedic Research Society. Published by Wiley Periodicals LLC.
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