3D magnetization transfer (MT) for the visualization of cardiac free-running Purkinje fibers: an ex vivo proof of concept.
Cardiac conduction system
Free-running Purkinje fibers
Magnetization transfer
Journal
Magma (New York, N.Y.)
ISSN: 1352-8661
Titre abrégé: MAGMA
Pays: Germany
ID NLM: 9310752
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
13
10
2020
accepted:
22
12
2020
revised:
02
12
2020
pubmed:
24
1
2021
medline:
5
10
2021
entrez:
23
1
2021
Statut:
ppublish
Résumé
We investigate the possibility to exploit high-field MRI to acquire 3D images of Purkinje network which plays a crucial role in cardiac function. Since Purkinje fibers (PF) have a distinct cellular structure and are surrounded by connective tissue, we investigated conventional contrast mechanisms along with the magnetization transfer (MT) imaging technique to improve image contrast between ventricular structures of differing macromolecular content. Three fixed porcine ventricular samples were used with free-running PFs on the endocardium. T1, T2*, T2, and M0 were evaluated on 2D slices for each sample at 9.4 T. MT parameters were optimized using hard pulses with different amplitudes, offset frequencies and durations. The cardiac structure was assessed through 2D and 3D T1w images with isotropic resolutions of 150 µm. Histology, immunofluorescence, and qPCR were performed to analyze collagen contents of cardiac tissue and PF. An MT preparation module of 350 ms duration inserted into the sequence with a B1 = 10 µT and frequency offset = 3000 Hz showed the best contrast, approximately 0.4 between PFs and myocardium. Magnetization transfer ratio (MTR) appeared higher in the cardiac tissue (MTR = 44.7 ± 3.5%) than in the PFs (MTR = 25.2 ± 6.3%). MT significantly improves contrast between PFs and ventricular myocardium and appears promising for imaging the 3D architecture of the Purkinje network.
Identifiants
pubmed: 33484367
doi: 10.1007/s10334-020-00905-w
pii: 10.1007/s10334-020-00905-w
pmc: PMC8338918
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
605-618Subventions
Organisme : National Research Agency ANR
ID : 10-IAHU04-LIRYC
Organisme : Region Nouvelle Nouvelle Aquitaine
ID : 2016-0127 Purkinje
Organisme : European Research Council
ID : 322886-SYMPHONY
Pays : International
Informations de copyright
© 2021. The Author(s).
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