Protocol for pressure-clamped patch-clamp recording at the node of Ranvier of rat myelinated nerves.

Action Potentials Animals Nerve Fibers, Myelinated / metabolism Patch-Clamp Techniques Ranvier's Nodes / metabolism Rats
Cell membrane Model organisms Neuroscience

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
19 03 2021
Historique:
entrez: 25 1 2021
pubmed: 26 1 2021
medline: 26 1 2021
Statut: epublish

Résumé

The patch-clamp recording technique is indispensable for studying ion channel functions of cells but is challenging to apply to the node of Ranvier, a key site where action potentials are conducted along myelinated nerves. We have developed a pressure-clamped patch-clamp recording method applying to the node of Ranvier of rat myelinated nerves. The step-by-step protocol described here allows researchers to apply this approach to study mechanisms underlying saltatory conduction and information processing in myelinated nerves of mammals. For complete information on the generation and use of this protocol, please refer to Kanda et al. (2019).

Identifiants

DOI: 10.1016/j.xpro.2020.100266 PMID: 33490982 PMC: PMC7806518
pubmed: 33490982
doi: 10.1016/j.xpro.2020.100266
pii: S2666-1667(20)30253-7
pmc: PMC7806518
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Pagination

100266

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS109059
Pays : United States

Informations de copyright

© 2020 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Neuron. 2019 Dec 4;104(5):960-971.e7
pubmed: 31630908

Auteurs

Hirosato Kanda (H)

Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Department of Pharmacology, Hyogo University of Health Sciences, Kobe, Hyogo 650-8530, Japan.

Sotatsu Tonomura (S)

Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Yi Dai (Y)

Department of Pharmacology, Hyogo University of Health Sciences, Kobe, Hyogo 650-8530, Japan.

Jianguo G Gu (JG)

Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système nerveux › Potentiels de membrane › Potentiels d'action › Protocol for pressure-clamped patch-clamp...
questionsmedicales.fr › Eucaryotes › Animaux › Protocol for pressure-clamped patch-clamp...
questionsmedicales.fr › Cellules › Neurones › Neurofibres myélinisées › Protocol for pressure-clamped patch-clamp...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Techniques de patch-clamp › Protocol for pressure-clamped patch-clamp...
questionsmedicales.fr › Cellules › Neurones › Neurofibres › Gaine de myéline › Noeuds de Ranvier › Protocol for pressure-clamped patch-clamp...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Protocol for pressure-clamped patch-clamp...