Nuclear magnetic resonance immunoassay of tetanus antibodies based on the displacement of magnetic nanoparticles.
Animals
Antibodies, Immobilized
/ chemistry
Antibodies, Monoclonal
/ chemistry
Enzyme-Linked Immunosorbent Assay
/ instrumentation
Humans
Magnetic Resonance Spectroscopy
/ instrumentation
Magnetite Nanoparticles
/ chemistry
Prostate-Specific Antigen
/ blood
Rabbits
Streptavidin
/ chemistry
Tetanus Toxoid
/ blood
Assay
Elution
Magnetic nanoparticles
Relaxometry
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
29
10
2019
accepted:
03
12
2020
revised:
11
11
2020
pubmed:
26
1
2021
medline:
2
7
2021
entrez:
25
1
2021
Statut:
ppublish
Résumé
A nuclear magnetic resonance (NMR) immunoassay based on the application of carbon-coated iron nanoparticles conjugated with recognition molecules was designed. The principle of the assay is that ELISA plates are coated with a capture element, and then an analyte is added and detected by conjugating the magnetic nanoparticles with recognition molecules. Afterwards, the elution solution (0.1-M sodium hydroxide) is added to displace the magnetic nanoparticles from the well surfaces into the solution. The detached magnetic nanoparticles reduce transverse relaxation time (T2) values of protons from the surrounding solution. A portable NMR relaxometer is used to measure the T2. Magnetic nanoparticles conjugated with streptavidin, monoclonal antibodies, and protein G were applied for the detection of biotinylated albumin, prostate-specific antigen, and IgG specific to tetanus toxoid (TT). The limit of detection of anti-TT IgG was 0.08-0.12 mIU/mL. The reproducibility of the assay was within the acceptable range (CV < 7.4%). The key novelty of the immunoassay is that the displacement of the nanoparticles from the solid support by the elution solution allows the advantages of the solid phase assay to be combined with the sensitive detection of the T2 changes in a volume of liquid.
Identifiants
pubmed: 33491121
doi: 10.1007/s00216-020-03112-7
pii: 10.1007/s00216-020-03112-7
doi:
Substances chimiques
Antibodies, Immobilized
0
Antibodies, Monoclonal
0
Magnetite Nanoparticles
0
Tetanus Toxoid
0
Streptavidin
9013-20-1
Prostate-Specific Antigen
EC 3.4.21.77
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1461-1471Subventions
Organisme : Russian Science Foundation
ID : 17-15-01116
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