Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.
Amino Acid Sequence
Cell Cycle Proteins
/ metabolism
Centrioles
/ metabolism
Centrosome
/ metabolism
Chromosome Segregation
HEK293 Cells
HeLa Cells
Humans
Interphase
Microtubule-Associated Proteins
/ chemistry
Models, Biological
Nuclear Proteins
/ chemistry
Phenotype
Protein Serine-Threonine Kinases
/ metabolism
Protein Transport
Proto-Oncogene Proteins
/ metabolism
Sequence Homology, Amino Acid
Spindle Apparatus
/ metabolism
Polo-Like Kinase 1
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 03 2021
01 03 2021
Historique:
received:
21
05
2020
revised:
27
10
2020
accepted:
15
12
2020
entrez:
25
1
2021
pubmed:
26
1
2021
medline:
10
9
2021
Statut:
ppublish
Résumé
Centrioles duplicate in interphase only once per cell cycle. Newly formed centrioles remain associated with their mother centrioles. The two centrioles disengage at the end of mitosis, which licenses centriole duplication in the next cell cycle. Therefore, timely centriole disengagement is critical for the proper centriole duplication cycle. However, the mechanisms underlying centriole engagement during interphase are poorly understood. Here, we show that Cep57 and Cep57L1 cooperatively maintain centriole engagement during interphase. Codepletion of Cep57 and Cep57L1 induces precocious centriole disengagement in interphase without compromising cell cycle progression. The disengaged daughter centrioles convert into centrosomes during interphase in a Plk1-dependent manner. Furthermore, the centrioles reduplicate and the centriole number increases, which results in chromosome segregation errors. Overall, these findings demonstrate that the maintenance of centriole engagement by Cep57 and Cep57L1 during interphase is crucial for the tight control of centriole copy number and thus for proper chromosome segregation.
Identifiants
pubmed: 33492359
pii: 211711
doi: 10.1083/jcb.202005153
pmc: PMC7836272
pii:
doi:
Substances chimiques
CEP57 protein, human
0
Cell Cycle Proteins
0
Microtubule-Associated Proteins
0
Nuclear Proteins
0
Proto-Oncogene Proteins
0
Protein Serine-Threonine Kinases
EC 2.7.11.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021 Ito et al.
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