HCMV-controlling NKG2C
Biomarkers
Cell Differentiation
Cytokines
/ metabolism
Cytomegalovirus
/ immunology
Cytomegalovirus Infections
/ immunology
Hematopoietic Stem Cells
/ cytology
Host-Pathogen Interactions
/ immunology
Humans
Immunophenotyping
Inflammation Mediators
/ metabolism
Killer Cells, Natural
/ immunology
Lymphocyte Activation
/ immunology
NK Cell Lectin-Like Receptor Subfamily C
/ metabolism
HCMV
HCV
HIV
Lin(−)CD34(+)DNAM-1(bright)
NKG2C
Natural killer cells
common lymphocyte precursors
hematopoietic stem cells
viral infection
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
03
06
2020
revised:
26
11
2020
accepted:
04
12
2020
pubmed:
26
1
2021
medline:
5
10
2021
entrez:
25
1
2021
Statut:
ppublish
Résumé
There is limited knowledge on the origin and development from CD34 This study sought to characterize the NK-cell progeny of CD34 Highly purified precursors were obtained by flow cytometric sorting and cultured in standard NK-cell differentiation media (ie, SCF, FLT3, IL-7, IL-15). Phenotypic and functional analyses on progenies were performed by multiparametric cytofluorimetric assays. Transcriptional signatures of NK-cell progenies were studied by microarray analysis. Inhibition of cytomegalovirus replication was studied by PCR. Unlike conventional CD34 During viral infections, precursors of adaptive NK cells are released and circulate in the peripheral blood.
Sections du résumé
BACKGROUND
There is limited knowledge on the origin and development from CD34
OBJECTIVE
This study sought to characterize the NK-cell progeny of CD34
METHODS
Highly purified precursors were obtained by flow cytometric sorting and cultured in standard NK-cell differentiation media (ie, SCF, FLT3, IL-7, IL-15). Phenotypic and functional analyses on progenies were performed by multiparametric cytofluorimetric assays. Transcriptional signatures of NK-cell progenies were studied by microarray analysis. Inhibition of cytomegalovirus replication was studied by PCR.
RESULTS
Unlike conventional CD34
CONCLUSIONS
During viral infections, precursors of adaptive NK cells are released and circulate in the peripheral blood.
Identifiants
pubmed: 33493558
pii: S0091-6749(21)00087-7
doi: 10.1016/j.jaci.2020.12.648
pii:
doi:
Substances chimiques
Biomarkers
0
Cytokines
0
Inflammation Mediators
0
KLRC2 protein, human
0
NK Cell Lectin-Like Receptor Subfamily C
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2343-2357Informations de copyright
Copyright © 2021. Published by Elsevier Inc.