GC-MS/MS analysis of metabolites derived from a single human blastocyst.
Branched-chain amino acids
Culture medium
Embryo quality
Metabolomics
Journal
Metabolomics : Official journal of the Metabolomic Society
ISSN: 1573-3890
Titre abrégé: Metabolomics
Pays: United States
ID NLM: 101274889
Informations de publication
Date de publication:
25 01 2021
25 01 2021
Historique:
received:
14
09
2020
accepted:
05
01
2021
entrez:
26
1
2021
pubmed:
27
1
2021
medline:
21
9
2021
Statut:
epublish
Résumé
The field of assisted reproductive technology (ART) has significantly advanced; however, morphological evaluation remains as the chosen method of assessment of embryo quality. We aimed to examine metabolic changes in embryo culture medium to develop a non-invasive method for evaluation of embryo quality. We performed metabolic analysis of culture medium obtained from a single blastocyst cultured for freezing. In total, 187 (39.8%) of the 469 detectable organic acid metabolites were identified. A significant change (p < 0.05) was observed in eight metabolites between the good-quality and poor-quality embryo groups. Differences were observed in several metabolic pathways between the good-quality and poor-quality embryo groups. Metabolites that showed significant changes were primarily involved in the metabolism of branched-chain amino acids. The quantification of metabolism in human embryos may assist in identification and selection of good-quality embryos with high rates of survival before freezing and implantation in conjunction with morphological classification. This may help to identify embryos with high rates of survival.
Identifiants
pubmed: 33495963
doi: 10.1007/s11306-021-01770-x
pii: 10.1007/s11306-021-01770-x
pmc: PMC7835178
doi:
Substances chimiques
Amino Acids, Branched-Chain
0
Culture Media
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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