Asymmetrizing an icosahedral virus capsid by hierarchical assembly of subunits with designed asymmetry.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 01 2021
26 01 2021
Historique:
received:
18
06
2020
accepted:
07
12
2020
entrez:
27
1
2021
pubmed:
28
1
2021
medline:
7
2
2021
Statut:
epublish
Résumé
Symmetrical protein complexes are ubiquitous in biology. Many have been re-engineered for chemical and medical applications. Viral capsids and their assembly are frequent platforms for these investigations. A means to create asymmetric capsids may expand applications. Here, starting with homodimeric Hepatitis B Virus capsid protein, we develop a heterodimer, design a hierarchical assembly pathway, and produce asymmetric capsids. In the heterodimer, the two halves have different growth potentials and assemble into hexamers. These preformed hexamers can nucleate co-assembly with other dimers, leading to Janus-like capsids with a small discrete hexamer patch. We can remove the patch specifically and observe asymmetric holey capsids by cryo-EM reconstruction. The resulting hole in the surface can be refilled with fluorescently labeled dimers to regenerate an intact capsid. In this study, we show how an asymmetric subunit can be used to generate an asymmetric particle, creating the potential for a capsid with different surface chemistries.
Identifiants
pubmed: 33500404
doi: 10.1038/s41467-020-20862-1
pii: 10.1038/s41467-020-20862-1
pmc: PMC7838286
doi:
Substances chimiques
Capsid Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
589Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM129354
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI144022
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI118933
Pays : United States
Références
Biochemistry. 1999 Nov 2;38(44):14644-52
pubmed: 10545189
J Biol Chem. 2015 Jun 26;290(26):16238-45
pubmed: 25953902
Nature. 2008 Jun 26;453(7199):1262-5
pubmed: 18563089
Biochemistry. 2009 Mar 3;48(8):1736-42
pubmed: 19196007
Science. 2012 Jun 1;336(6085):1171-4
pubmed: 22654060
Nanoscale. 2011 Jun;3(6):2376-89
pubmed: 21461437
J Virol. 1992 Jan;66(1):106-14
pubmed: 1370083
Nature. 2016 Sep 14;537(7620):320-7
pubmed: 27629638
Elife. 2016 Nov 15;5:
pubmed: 27845625
J Am Soc Mass Spectrom. 2019 Jun;30(6):898-904
pubmed: 30993638
ACS Chem Biol. 2020 Dec 18;15(12):3124-3132
pubmed: 32459465
Nature. 2016 Jul 7;535(7610):136-9
pubmed: 27309817
Annu Rev Biophys Biomol Struct. 2000;29:105-53
pubmed: 10940245
Nat Chem. 2018 Jul;10(7):732-739
pubmed: 29713036
J Struct Biol. 2007 Jan;157(1):38-46
pubmed: 16859925
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14243-8
pubmed: 8962033
Nature. 1997 Mar 6;386(6620):91-4
pubmed: 9052787
Biotechnol Bioeng. 2014 Dec;111(12):2398-406
pubmed: 24917327
Annu Rev Biophys. 2017 May 22;46:23-42
pubmed: 28301774
Sci Rep. 2011;1:5
pubmed: 22355524
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1915-20
pubmed: 10051569
J Am Soc Mass Spectrom. 2018 Nov;29(11):2160-2172
pubmed: 30112619
J Struct Biol. 2015 Nov;192(2):216-21
pubmed: 26278980
Science. 2006 May 12;312(5775):873-5
pubmed: 16690856
Annu Rev Phytopathol. 2008;46:361-84
pubmed: 18473700
Protein Sci. 2017 Nov;26(11):2170-2180
pubmed: 28795465
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2217-21
pubmed: 11226219
Biomacromolecules. 2010 Oct 11;11(10):2804-9
pubmed: 20839852
J Virol. 2019 Jun 28;93(14):
pubmed: 31043524
J Allergy Clin Immunol. 2005 Oct;116(4):893-9
pubmed: 16210066
Adv Mater. 2010 Mar 12;22(10):1060-71
pubmed: 20401930
Nanomedicine (Lond). 2011 Feb;6(2):351-64
pubmed: 21385137
Nature. 2012 Nov 22;491(7425):578-81
pubmed: 23172215
Science. 2004 Oct 15;306(5695):419-20
pubmed: 15486279
J Am Chem Soc. 2012 Nov 14;134(45):18506-9
pubmed: 23101937
Nat Biotechnol. 2006 Mar;24(3):358-62
pubmed: 16474383
Mol Cell. 1999 Jun;3(6):771-80
pubmed: 10394365
Cell. 1994 Jun 17;77(6):943-50
pubmed: 8004680
Nat Nanotechnol. 2016 Mar;11(3):295-303
pubmed: 26689376
J Am Chem Soc. 2020 Apr 29;142(17):7868-7882
pubmed: 32233479
PLoS One. 2015 Apr 01;10(4):e0120751
pubmed: 25830365
Annu Rev Phys Chem. 2015 Apr;66:217-39
pubmed: 25532951
Viruses. 2018 Jan 04;10(1):
pubmed: 29300359
J Am Soc Mass Spectrom. 2020 Jan 2;31(1):146-154
pubmed: 32881508
Chem Soc Rev. 2018 May 21;47(10):3543-3557
pubmed: 29714396
Proc Natl Acad Sci U S A. 2008 Oct 21;105(42):16148-52
pubmed: 18849473
Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):2897-902
pubmed: 24516140
Curr Opin Struct Biol. 2019 Apr;55:77-84
pubmed: 31005680
J Am Chem Soc. 2014 Mar 5;136(9):3536-41
pubmed: 24548133
ACS Nano. 2015 Sep 22;9(9):9087-96
pubmed: 26266555
Nat Protoc. 2007;2(3):490-8
pubmed: 17406612
Curr Opin Biotechnol. 2017 Aug;46:57-65
pubmed: 28160725
Science. 2016 May 6;352(6286):680-7
pubmed: 27151862
Science. 2012 Jun 1;336(6085):1129
pubmed: 22654051
Elife. 2018 Jan 29;7:
pubmed: 29377794
Trends Biochem Sci. 1995 Jul;20(7):285-6
pubmed: 7667882
ACS Appl Mater Interfaces. 2013 Mar;5(6):1857-69
pubmed: 23394306
J Virol. 2019 Apr 17;93(9):
pubmed: 30787152
Nature. 2017 Dec 21;552(7685):415-420
pubmed: 29236688
Intervirology. 2001;44(2-3):98-114
pubmed: 11509871
Nat Chem. 2019 Mar;11(3):204-212
pubmed: 30643229
Anal Chem. 2018 Jun 19;90(12):7267-7274
pubmed: 29708733
J Virol. 2010 Feb;84(3):1607-15
pubmed: 19939922
J Am Chem Soc. 2012 May 30;134(21):8823-30
pubmed: 22329660
Anal Chem. 2019 Jan 2;91(1):622-636
pubmed: 30383361
Trends Microbiol. 2011 Jan;19(1):14-23
pubmed: 21163649
J Struct Biol. 2017 Apr;198(1):38-42
pubmed: 28254381
Curr Opin Chem Biol. 2017 Jun;38:108-116
pubmed: 28426952
Chem Soc Rev. 2016 Jul 25;45(15):4074-126
pubmed: 27152673
Curr Biol. 2009 Jan 13;19(1):R25-6
pubmed: 19138586
J Am Soc Mass Spectrom. 2018 Oct;29(10):2086-2095
pubmed: 29987663
J Biol Chem. 2003 May 16;278(20):18249-55
pubmed: 12639968
J Am Chem Soc. 2017 Nov 22;139(46):16932-16938
pubmed: 29125756