Clonal hematopoiesis in adult pure red cell aplasia.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
26 01 2021
Historique:
received: 27 09 2020
accepted: 12 01 2021
entrez: 27 1 2021
pubmed: 28 1 2021
medline: 16 9 2021
Statut: epublish

Résumé

Idiopathic pure red cell aplasia (PRCA) and secondary PRCA associated with thymoma and large granular lymphocyte leukemia are generally considered to be immune-mediated. The PRCA2004/2006 study showed that poor responses to immunosuppression and anemia relapse were associated with death. PRCA may represent the prodrome to MDS. Thus, clonal hematopoiesis may be responsible for treatment failure. We investigated gene mutations in myeloid neoplasm-associated genes in acquired PRCA. We identified 21 mutations affecting amino acid sequences in 11 of the 38 adult PRCA patients (28.9%) using stringent filtering of the error-prone sequences and SNPs. Four PRCA patients showed 7 driver mutations in TET2, DNMT3A and KDM6A, and 2 PRCA patients carried multiple mutations in TET2. Five PRCA patients had mutations with high VAFs exceeding 0.3. These results suggest that clonal hematopoiesis by stem/progenitor cells might be related to the pathophysiology of chronic PRCA in certain adult patients.

Identifiants

pubmed: 33500526
doi: 10.1038/s41598-021-81890-5
pii: 10.1038/s41598-021-81890-5
pmc: PMC7838416
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2253

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Auteurs

Naohito Fujishima (N)

Division of Blood Transfusion, 44-2 Hiroomote Aza Hasunuma, Akita, 010-8543, Japan. naofuji@doc.med.akita-u.ac.jp.

Junki Kohmaru (J)

Akita University Bioscience Education and Research Center, Akita, Japan.

Souichi Koyota (S)

Akita University Bioscience Education and Research Center, Akita, Japan.

Keiji Kuba (K)

Akita University Bioscience Education and Research Center, Akita, Japan.
Department of Biochemistry and Metabolic Science, Akita University Graduate School of Medicine, Akita, Japan.

Tomoo Saga (T)

Department of General Internal Medicine and Clinical Laboratory Medicine, Akita University Graduate School of Medicine, Akita, Japan.

Ayumi Omokawa (A)

Department of General Internal Medicine and Clinical Laboratory Medicine, Akita University Graduate School of Medicine, Akita, Japan.

Yuki Moritoki (Y)

Department of General Internal Medicine and Clinical Laboratory Medicine, Akita University Graduate School of Medicine, Akita, Japan.

Shigeharu Ueki (S)

Department of General Internal Medicine and Clinical Laboratory Medicine, Akita University Graduate School of Medicine, Akita, Japan.

Fumihiro Ishida (F)

Department of Biomedical Laboratory Sciences, Shinshu University School of Medicine, Matsumoto, Japan.

Shinji Nakao (S)

Department of Cellular Transplantation Biology, Kanazawa University Graduate School of Medicine, Kanazawa, Japan.

Akira Matsuda (A)

Department of Hemato-Oncology, International Medical Center, Saitama Medical University, Saitama, Japan.

Akiko Ohta (A)

Division of Public Health, Department of Social Medicine, Saitama Medical University, Saitama, Japan.

Kaoru Tohyama (K)

Department of Laboratory Medicine, Kawasaki Medical School, Okayama, Japan.

Hiroshi Yamasaki (H)

Department of Hematology and Oncology, Kumamoto City Hospital, Kumamoto, Japan.

Kensuke Usuki (K)

Department of Hematology, NTT Medical Center Tokyo, Tokyo, Japan.

Yasuhiro Nakashima (Y)

Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Shinya Sato (S)

Department of Hematology, Atomic Bomb Disease and Hibakusha Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan.

Yasushi Miyazaki (Y)

Department of Hematology, Atomic Bomb Disease and Hibakusha Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan.

Yasuhito Nannya (Y)

Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Seishi Ogawa (S)

Department of Pathology and Tumor Biology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Kenichi Sawada (K)

Department of Hematology, Nephrology and Rheumatology, Akita University Graduate School of Medicine, Akita, Japan.

Kinuko Mitani (K)

Department of Hematology and Oncology, Dokkyo Medical University, Tochigi, Japan.

Makoto Hirokawa (M)

Department of General Internal Medicine and Clinical Laboratory Medicine, Akita University Graduate School of Medicine, Akita, Japan. makhirokawa08@gmail.com.

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