Small nucleolar RNA of silkworm can translocate from the nucleolus to the cytoplasm under abiotic stress.
UV radiation
nucleocytoplasmic distribution
serum starvation
small nucleolar RNA
Journal
Cell biology international
ISSN: 1095-8355
Titre abrégé: Cell Biol Int
Pays: England
ID NLM: 9307129
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
received:
15
12
2020
accepted:
24
01
2021
pubmed:
28
1
2021
medline:
9
11
2021
entrez:
27
1
2021
Statut:
ppublish
Résumé
Small nucleolar RNAs (snoRNAs) are thought to be exclusively nuclear and guide nucleotide modifications of ribosomal RNAs. Recently, more and more evidence has suggested that the nucleolus is a stress sensor for changes in growth status and that snoRNAs may orchestrate the response to environmental stress through molecular interactions outside of the nucleus. We previously showed that a box C/D snoRNA Bm-15 had both nuclear and cytoplasmic location in BmN4 cell line of the silkworm, Bombyx mori. To further study the functional roles of Bm-15, changes in expression level and cellular location of Bm-15 were examined in BmN4 cells subjected to serum starvation and ultraviolet (UV) ray radiation. Results indicated that total RNA level of Bm-15 was unchanged after 24 h serum starvation, but exhibited 3-fold increases in the cytoplasm, and the nuclear-to-cytosolic distribution ratio was reduced from 5:1 to 2:1. Moreover, UV radiation also causes rapid decline in nuclear Bm-15 and progressive cytoplasmic accumulation with a percentage of 22% and 57% after 6 and 24 h UV radiation. UV treatment results in a dramatic decrease in Bm-15 nuclear-to-cytosolic ratio from 7:1 to 2:1 and 2:1 to 1:20 after 6 and 24 h UV radiation, respectively. We show here for the first time that box C/D snoRNAs can translocate from the nucleus to the cytoplasm under the abiotic stress of nutritional deficiency and UV radiation. The rapid translocation of snoRNAs from nucleus to cytoplasm may slow down the maturation of rRNAs and synthesis of ribosomes to enhance the stress resistance of cells.
Substances chimiques
RNA, Small Nucleolar
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1091-1097Subventions
Organisme : National Natural Science Foundation of China
ID : 31100938 31970480
Organisme : Young Elite Scientist Sponsorship Program by China Association for Science and Technology
ID : YESS 20150026
Organisme : Training Program for Young Scholars sponsored by Nanyang Normal University
ID : nynuxjqg-2019-03
Informations de copyright
© 2021 International Federation for Cell Biology.
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