Nanostraw-Assisted Cellular Injection of Fluorescent Nanodiamonds via Direct Membrane Opening.
STED microscopy
cell transfection
electroporation
nanodiamonds
nanostraws
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
14
10
2020
revised:
16
12
2020
pubmed:
28
1
2021
medline:
10
7
2021
entrez:
27
1
2021
Statut:
ppublish
Résumé
Due to their stable fluorescence, biocompatibility, and amenability to functionalization, fluorescent nanodiamonds (FND) are promising materials for long term cell labeling and tracking. However, transporting them to the cytosol remains a major challenge, due to low internalization efficiencies and endosomal entrapment. Here, nanostraws in combination with low voltage electroporation pulses are used to achieve direct delivery of FND to the cytosol. The nanostraw delivery leads to efficient and rapid FND transport into cells compared to when incubating cells in a FND-containing medium. Moreover, whereas all internalized FND delivered by incubation end up in lysosomes, a significantly larger proportion of nanostraw-injected FND are in the cytosol, which opens up for using FND as cellular probes. Furthermore, in order to answer the long-standing question in the field of nano-biology regarding the state of the cell membrane on hollow nanostructures, live cell stimulated emission depletion (STED) microscopy is performed to image directly the state of the membrane on nanostraws. The time-lapse STED images reveal that the cell membrane opens entirely on top of nanostraws upon application of gentle electrical pulses, which supports the hypothesis that many FND are delivered directly to the cytosol, avoiding endocytosis and lysosomal entrapment.
Identifiants
pubmed: 33502091
doi: 10.1002/smll.202006421
doi:
Substances chimiques
Fluorescent Dyes
0
Nanodiamonds
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2006421Informations de copyright
© 2021 The Authors. Small published by Wiley-VCH GmbH.
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