Incorporation of dimethyl sulfoxide into experimental hydrophilic and hydrophobic adhesive resins: evaluation of cytotoxic activities.
dentin
eluates cytotoxicity
hydrophilic resin
hydrophobic resin
transdentinal
Journal
European journal of oral sciences
ISSN: 1600-0722
Titre abrégé: Eur J Oral Sci
Pays: England
ID NLM: 9504563
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
30
07
2020
revised:
17
11
2020
accepted:
18
11
2020
pubmed:
30
1
2021
medline:
27
4
2021
entrez:
29
1
2021
Statut:
ppublish
Résumé
This study evaluated the cytotoxicity of methacrylate-based resins containing dimethyl sulfoxide (DMSO). DMSO was incorporated into hydrophobic (R2) and hydrophilic (R5) resins at weight concentrations of 0, 0.01, 0.1, 1, 5, or 10 w/w %. Resin discs (n = 10/group) were prepared. Human gingival fibroblasts (HGF-1) were exposed to resin eluates for 24 h. Furthermore, dentin barrier test was performed using 3-D cultures of odontoblast-like cells (SV40 transfected pulp derived cells) with dentin slices of 400 µm thickness (n = 8). After acid etching of dentin, DMSO-modified resins were applied into the cavity part of the device and light-cured for 20 s. Cell viability (%) was assessed by MTT and analyzed spectrometrically. Data were analyzed by ANOVA and Tukey test (α = 0.05). Resin eluates showed statistically significantly lower % cell viability for all neat and DMSO-modified resins than seen for the negative control. Moreover, DMSO-R5 eluates resulted in significantly lower % cell viability than DMSO-R2 emulates. The dentin barrier test showed that DMSO-R2 did not result in significantly lower % cell viability, whereas incorporation of 1-10 w/w % DMSO into R5 resulted in significantly lower % of cell viability. Incorporating DMSO into hydrophilic self-etching resins may increase cytotoxicity. The biocompatibility is not influenced by the addition of DMSO into hydrophobic resin.
Substances chimiques
Composite Resins
0
Dental Cements
0
Dentin-Bonding Agents
0
Methacrylates
0
Resin Cements
0
Dimethyl Sulfoxide
YOW8V9698H
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12756Informations de copyright
© 2021 European Journal of Oral Sciences.
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