Epithelial-to-mesenchymal Transition Heterogeneity of Circulating Tumor Cells and Their Correlation With MDSCs and Tregs in HER2-negative Metastatic Breast Cancer Patients.
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor
/ blood
Breast Neoplasms
/ blood
Case-Control Studies
Epithelial-Mesenchymal Transition
Female
Flow Cytometry
Humans
Immunophenotyping
Middle Aged
Myeloid-Derived Suppressor Cells
/ immunology
Neoplastic Cells, Circulating
/ immunology
Neoplastic Stem Cells
/ immunology
Phenotype
T-Lymphocytes, Regulatory
/ immunology
Tumor Escape
CTC
EMT
MDSCs
Tregs
breast cancer
cancer stem cells
immune cells
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
04
12
2020
revised:
03
01
2021
accepted:
14
01
2021
entrez:
31
1
2021
pubmed:
1
2
2021
medline:
9
2
2021
Statut:
ppublish
Résumé
To investigate the correlation between circulating tumor cells (CTCs) bearing cancer stem cell (CSC) and epithelial-to-mesenchymal (EMT) phenotypes and the different immunosuppressive cells in peripheral blood of patients with metastatic breast cancer (mBC). Blood was obtained from 38 pre-treated patients with mBC before a new line of treatment. CTC detection and characterization was performed by triple immunofluorescent staining, while Myeloid-derived Suppressor Cells (MDSCs) and T regulatory cells (Tregs) were analyzed by multi-flow cytometry. CTCs were detected in 16 (42.1%) of patients. Based on the co-expression of ALDH1, TWIST and CK, CTCs revealed an important heterogeneity: CTCs with a CSC/partial-EMT, CSC/Epithelial-like, non-CSC/partial-EMT and non-CSC/Epithelial-like phenotype were detected in 7 (18.4%), 7 (18.4%), 1 (1.4%) and 9 (23.7%) of patients, respectively. Immunophenotyping of MDSCs identified 2 monocytic [M-MDSCs; CD14 These findings provide evidence that CTCs in ER
Sections du résumé
BACKGROUND
BACKGROUND
To investigate the correlation between circulating tumor cells (CTCs) bearing cancer stem cell (CSC) and epithelial-to-mesenchymal (EMT) phenotypes and the different immunosuppressive cells in peripheral blood of patients with metastatic breast cancer (mBC).
MATERIALS AND METHODS
METHODS
Blood was obtained from 38 pre-treated patients with mBC before a new line of treatment. CTC detection and characterization was performed by triple immunofluorescent staining, while Myeloid-derived Suppressor Cells (MDSCs) and T regulatory cells (Tregs) were analyzed by multi-flow cytometry.
RESULTS
RESULTS
CTCs were detected in 16 (42.1%) of patients. Based on the co-expression of ALDH1, TWIST and CK, CTCs revealed an important heterogeneity: CTCs with a CSC/partial-EMT, CSC/Epithelial-like, non-CSC/partial-EMT and non-CSC/Epithelial-like phenotype were detected in 7 (18.4%), 7 (18.4%), 1 (1.4%) and 9 (23.7%) of patients, respectively. Immunophenotyping of MDSCs identified 2 monocytic [M-MDSCs; CD14
CONCLUSION
CONCLUSIONS
These findings provide evidence that CTCs in ER
Identifiants
pubmed: 33517270
pii: 41/2/661
doi: 10.21873/anticanres.14817
doi:
Substances chimiques
Biomarkers, Tumor
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
661-670Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.