Physicochemical considerations for bottom-up synthetic biology.
bottom-up synthetic cells
macromolecular crowding
membrane reconstitution
physicochemical homeostasis
synthetic biochemistry
vesicles
Journal
Emerging topics in life sciences
ISSN: 2397-8554
Titre abrégé: Emerg Top Life Sci
Pays: England
ID NLM: 101706399
Informations de publication
Date de publication:
11 11 2019
11 11 2019
Historique:
received:
28
06
2019
revised:
05
08
2019
accepted:
07
08
2019
entrez:
1
2
2021
pubmed:
11
11
2019
medline:
11
11
2019
Statut:
ppublish
Résumé
The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like systems require control of the internal pH, ionic strength, (macro)molecular crowding, redox state and metabolic energy conservation. These physicochemical parameters influence protein activity and need to be maintained within limits to ensure the system remains in steady-state. We present the physicochemical considerations for building synthetic cells with dimensions ranging from the smallest prokaryotes to eukaryotic cells.
Identifiants
pubmed: 33523159
pii: 220559
doi: 10.1042/ETLS20190017
pmc: PMC7289010
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
445-458Informations de copyright
© 2019 The Author(s).
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