Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration.
Cell culture
Cell isolation
Microscopy
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
19 03 2021
19 03 2021
Historique:
entrez:
3
2
2021
pubmed:
4
2
2021
medline:
4
2
2021
Statut:
epublish
Résumé
The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and
Identifiants
pubmed: 33532742
doi: 10.1016/j.xpro.2021.100298
pii: S2666-1667(21)00005-8
pmc: PMC7829339
doi:
Substances chimiques
Nicotinamide Mononucleotide
1094-61-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100298Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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