Observation of arenavirus nucleoprotein heptamer assembly.
Arenaviridae
Bunyavirales
assembly
nucleoprotein
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
revised:
18
01
2021
received:
17
12
2020
accepted:
01
02
2021
pubmed:
4
2
2021
medline:
7
1
2022
entrez:
3
2
2021
Statut:
ppublish
Résumé
Arenaviruses are enveloped viruses containing a segmented, negative, and ambisense single-stranded RNA genome wrapped with a nucleoprotein (NP). The NP is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The NP associates with RNA to form a ribonucleoprotein (RNP) complex, and this implies self-assembly while the exact structure of this polymer is not yet known. Here, we report a measurement of the full-length Mopeia virus NP by negative stain transmission electron microscopy. We observed RNP complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.
Identifiants
pubmed: 33534950
doi: 10.1002/2211-5463.13106
pmc: PMC8016135
doi:
Substances chimiques
Nucleoproteins
0
RNA-Binding Proteins
0
Recombinant Proteins
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1076-1083Informations de copyright
© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Références
Nucleic Acids Res. 2019 Jul 2;47(W1):W5-W10
pubmed: 31062021
J Struct Biol. 2007 Jan;157(1):38-46
pubmed: 16859925
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Methods Mol Biol. 2021;2231:203-224
pubmed: 33289895
Acta Crystallogr D Struct Biol. 2017 Aug 1;73(Pt 8):650-659
pubmed: 28777080
Arch Virol. 2019 Jul;164(7):1949-1965
pubmed: 31065850
Bull World Health Organ. 1977;55(4):441-4
pubmed: 304387
Genome Res. 2004 Jun;14(6):1188-90
pubmed: 15173120
Nature. 2010 Dec 9;468(7325):779-83
pubmed: 21085117
Med Microbiol Immunol. 1986;175(2-3):79-83
pubmed: 3724669
Nucleic Acids Res. 2003 Jul 1;31(13):3320-3
pubmed: 12824317
Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19365-70
pubmed: 22084115
J Gen Virol. 1983 Apr;64 (Pt 4):833-42
pubmed: 6682139
Arch Virol. 2018 Aug;163(8):2295-2310
pubmed: 29680923
Viruses. 2020 Jul 17;12(7):
pubmed: 32708976
J Biol Chem. 2011 Nov 4;286(44):38748-38756
pubmed: 21917929
PLoS Pathog. 2011 May;7(5):e1002030
pubmed: 21589902