Molecular and physiological analysis of indole-3-acetic acid degradation in Bradyrhizobium japonicum E109.

Bradyrhizobium / genetics Indoleacetic Acids / metabolism Indoles / metabolism Metabolic Networks and Pathways / genetics Tryptamines / metabolism
2-(2-Hydroperoxy-3-hydroxyindolin-3-yl) acetic acid 3-Indoleacetic acid 2 3-Oxide 3-Phenylpropionate dioxygenase B. japonicum Indole-3-acetic acid

Journal

Research in microbiology
ISSN: 1769-7123
Titre abrégé: Res Microbiol
Pays: France
ID NLM: 8907468

Informations de publication

Date de publication:
Historique:
received: 27 08 2020
revised: 20 01 2021
accepted: 26 01 2021
pubmed: 5 2 2021
medline: 25 2 2023
entrez: 4 2 2021
Statut: ppublish

Résumé

Bradyrhizobium japonicum E109 is a bacterium widely used for inoculants production in Argentina. It is known for its ability to produce several phytohormones and degrade indole-3-acetic acid (IAA). The genome sequence of B. japonicum E109 was recently analyzed and it showed the presence of genes related to the synthesis of IAA by indole-3-acetonitrile, indole-3-acetamide and tryptamine pathways. Nevertheless, B. japonicum E109 is not able to produce IAA and instead has the ability to degrade this hormone under saprophytic culture conditions. This work aimed to study the molecular and physiological features of IAA degradation and identify the genes responsible of this activity. In B. japonicum E109 we identified two sequences coding for a putative 3-phenylpropionate dioxygenase (subunits α and β) responsible for the IAA degradation that were homologous to the canonical cluster of iacC and iacD of Pseudomonas putida 1290. These genes form a separate cluster together with three additional genes with unknown functions. The degradation activity was found to be constitutively expressed in B. japonicum E109. As products of IAA degradation, we identified two compounds, 3-indoleacetic acid 2,3-oxide and 2-(2-hydroperoxy-3-hydroxyindolin-3-yl) acetic acid. Our report proposes, for the first time, a model for IAA degradation in Bradyrhizobium.

Identifiants

DOI: 10.1016/j.resmic.2021.103814 PMID: 33539931
pubmed: 33539931
pii: S0923-2508(21)00027-9
doi: 10.1016/j.resmic.2021.103814
pii:
doi:

Substances chimiques

Indoleacetic Acids 0
Indoles 0
Tryptamines 0
tryptamine 422ZU9N5TV
indoleacetic acid 6U1S09C61L
indoleacetamide 879-37-8
indole-3-acetonitrile AG97OFW8JW

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

103814

Informations de copyright

Copyright © 2021 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflict of interest.

Auteurs

Daniela Torres (D)

Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: dtorres@exa.unrc.edu.ar.

Elías Mongiardini (E)

Laboratorio de Interacciones Rizobios y Soja. Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas. Universidad Nacional de La Plata, La Plata, Argentina. Electronic address: mongiardini@biol.unlp.edu.ar.

Florencia Donadío (F)

Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: fdonadio@exa.unrc.edu.ar.

Raúl Donoso (R)

Programa Institucional de Fomento a la Investigación, Desarrollo e Innovación (PIDi), Universidad Tecnológica Metropolitana, Santiago, Chile; Center of Applied Ecology and Sustainability (CAPES), Santiago, Chile. Electronic address: radonoso@uai.cl.

Gonzalo Recabarren-Gajardo (G)

Bioactive Heterocycles Synthesis Laboratory, BHSL, Departamento de Farmacia, Facultad de Química y de Farmacia, Pontificia Universidad Católica de Chile, Santiago, Chile; Centro Interdisciplinario de Neurociencias, Pontificia Universidad Católica de Chile, Santiago, Chile. Electronic address: grecabarren@uc.cl.

José Gualpa (J)

Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: jgualpa@exa.unrc.edu.ar.

Stijn Spaepen (S)

Katholieke Universiteit Leuven. Leuven, Belgium.

Roberto Defez (R)

Institute of Biosciences and Bioresources, National Research Council of Italy, UOS Naples, Italy. Electronic address: roberto.defez@ibbr.cnr.it.

Gastón Lopez (G)

Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: glopez@exa.unrc.edu.ar.

Carmen Bianco (C)

Institute of Biosciences and Bioresources, National Research Council of Italy, UOS Naples, Italy. Electronic address: carmen.bianco@ibbr.cnr.it.

Fabricio Cassán (F)

Instituto de Investigaciones Agrobiotecnológicas (INIAB), Universidad Nacional de Río Cuarto, Córdoba Argentina. Electronic address: fcassan@exa.unrc.edu.ar.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Bradyrhizobiaceae › Bradyrhizobium › Molecular and physiological analysis of...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Acides indolacétiques › Molecular and physiological analysis of...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Molecular and physiological analysis of...
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Molecular and physiological analysis of...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Tryptamines › Molecular and physiological analysis of...