Ordered Clusters of the Complete Oxidative Phosphorylation System in Cardiac Mitochondria.
ATP synthase
cryo-electron microscopy
mitochondria
oxidative phosphorylation
respirasome
supercomplex
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Feb 2021
02 Feb 2021
Historique:
received:
22
12
2020
revised:
26
01
2021
accepted:
30
01
2021
entrez:
5
2
2021
pubmed:
6
2
2021
medline:
13
4
2021
Statut:
epublish
Résumé
The existence of a complete oxidative phosphorylation system (OXPHOS) supercomplex including both electron transport system and ATP synthases has long been assumed based on functional evidence. However, no structural confirmation of the docking between ATP synthase and proton pumps has been obtained. In this study, cryo-electron tomography was used to reveal the supramolecular architecture of the rat heart mitochondria cristae during ATP synthesis. Respirasome and ATP synthase structure in situ were determined using subtomogram averaging. The obtained reconstructions of the inner mitochondrial membrane demonstrated that rows of respiratory chain supercomplexes can dock with rows of ATP synthases forming oligomeric ordered clusters. These ordered clusters indicate a new type of OXPHOS structural organization. It should ensure the quickness, efficiency, and damage resistance of OXPHOS, providing a direct proton transfer from pumps to ATP synthase along the lateral pH gradient without energy dissipation.
Identifiants
pubmed: 33540542
pii: ijms22031462
doi: 10.3390/ijms22031462
pmc: PMC7867189
pii:
doi:
Substances chimiques
Proton Pumps
0
Mitochondrial Proton-Translocating ATPases
EC 3.6.3.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Foundation for Basic Research
ID : 19-04-00835
Organisme : National Research Center "Kurchatov Institute"
ID : thematic plan "Study of the processes of generation, transmission and distribution of energy in living organisms"
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