The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB.
3' Untranslated Regions
A549 Cells
Animals
Base Pairing
Computational Biology
/ methods
DNA Primers
/ chemical synthesis
DNA Transposable Elements
Enhancer Elements, Genetic
Genes, Reporter
Genome
Humans
Long Interspersed Nucleotide Elements
Luciferases
/ genetics
Macaca fascicularis
Mice
MicroRNAs
/ genetics
NF-kappa B
/ genetics
Pan troglodytes
Repressor Proteins
/ genetics
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 02 2021
04 02 2021
Historique:
received:
24
12
2019
accepted:
25
01
2021
entrez:
5
2
2021
pubmed:
6
2
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Transposable elements (TEs) are DNA sequences that cut or introduced into the genome, and they represent a massive portion of the human genome. TEs generate a considerable number of microRNAs (miRNAs) are derived from TEs (MDTEs). Numerous miRNAs are related to cancer, and hsa-miRNA-625 is a well-known oncomiR derived from long interspersed nuclear elements (LINEs). The relative expression of hsa-miRNA-625-5p differs in humans, chimpanzees, crab-eating monkeys, and mice, and four primers were designed against the 3'UTR of GATAD2B to analyze the different quantities of canonical binding sites and the location of miRNA binding sites. Luciferase assay was performed to score for the interaction between hsa-miRNA-625 and the 3'UTR of GATAD2B, while blocking NF-κB. In summary, the different numbers of canonical binding sites and the locations of miRNA binding sites affect gene expression, and NF-κB induces the enhancer activity of hsa-miRNA-625-5p by sharing the binding sites.
Identifiants
pubmed: 33542430
doi: 10.1038/s41598-021-82735-x
pii: 10.1038/s41598-021-82735-x
pmc: PMC7862687
doi:
Substances chimiques
3' Untranslated Regions
0
DNA Primers
0
DNA Transposable Elements
0
GATAD2B protein, human
0
MIRN625 microRNA, human
0
MicroRNAs
0
NF-kappa B
0
Repressor Proteins
0
Luciferases
EC 1.13.12.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3139Références
Nucleic Acids Res. 2011 May;39(9):3892-902
pubmed: 21245048
Int J Mol Med. 2016 Aug;38(2):446-56
pubmed: 27277384
Cell Res. 2016 Jun;26(6):655-73
pubmed: 27002217
Trends Genet. 2012 Dec;28(12):616-23
pubmed: 23040327
Proc Natl Acad Sci U S A. 1989 Apr;86(7):2336-40
pubmed: 2494664
Nucleic Acids Res. 2018 Jan 4;46(D1):D762-D769
pubmed: 29106570
FEBS Lett. 2014 Mar 18;588(6):915-21
pubmed: 24508466
Biochem Biophys Res Commun. 2016 Feb 19;470(4):838-44
pubmed: 26806308
Cell. 2018 Feb 8;172(4):650-665
pubmed: 29425488
J Mol Biol. 2005 Dec 9;354(4):994-1007
pubmed: 16288912
Cell. 2017 Mar 9;168(6):1000-1014.e15
pubmed: 28283057
RNA. 2004 Oct;10(10):1507-17
pubmed: 15383676
Nature. 2006 Oct 5;443(7111):521-4
pubmed: 17024082
Proc Natl Acad Sci U S A. 1988 Mar;85(5):1482-6
pubmed: 3125549
PLoS One. 2015 Jun 26;10(6):e0131365
pubmed: 26115450
Nucleic Acids Res. 2019 Jan 8;47(D1):D155-D162
pubmed: 30423142
Bioinformatics. 2018 Jun 1;34(11):1937-1938
pubmed: 29360956
Oncogene. 2015 Feb 19;34(8):965-77
pubmed: 24632613
Genome Biol. 2018 Nov 19;19(1):199
pubmed: 30454069
PLoS Genet. 2019 Mar 13;15(3):e1008036
pubmed: 30865625
Sci Rep. 2019 Sep 30;9(1):14007
pubmed: 31570746
Mol Biol Evol. 2015 Mar;32(3):623-8
pubmed: 25414126
Genome Res. 2009 Jul;19(7):1175-83
pubmed: 19336450
J Surg Oncol. 2013 Sep;108(4):230-5
pubmed: 23861214
Trends Genet. 2005 Jun;21(6):322-6
pubmed: 15922829
Nat Rev Genet. 2008 May;9(5):397-405
pubmed: 18368054
Biomed Res Int. 2014;2014:436097
pubmed: 24800225
Nat Rev Genet. 2009 Oct;10(10):691-703
pubmed: 19763152
Nat Rev Mol Cell Biol. 2005 May;6(5):376-85
pubmed: 15852042
Int J Biochem Cell Biol. 1997 Dec;29(12):1305-12
pubmed: 9570129
Nucleic Acids Res. 2016 Jul 8;44(W1):W147-53
pubmed: 27190236
Mob Genet Elements. 2014 May 22;4:e29255
pubmed: 25054081
Genome Res. 2013 Apr;23(4):604-15
pubmed: 23335364
Acta Biochim Biophys Sin (Shanghai). 2014 Nov;46(11):991-6
pubmed: 25274328
Nucleic Acids Res. 2001 Jan 1;29(1):281-3
pubmed: 11125113
PLoS Biol. 2005 Mar;3(3):e85
pubmed: 15723116
Science. 1991 Feb 15;251(4995):753
pubmed: 1990437
Genome Dyn. 2006;1:104-115
pubmed: 18724056
Science. 2016 Feb 12;351(6274):aac7247
pubmed: 26912865
Cell. 1987 Jan 16;48(1):121-8
pubmed: 3098435
Mol Cell Biol. 1990 May;10(5):2327-34
pubmed: 2183031
Genomics. 2009 Dec;94(6):397-406
pubmed: 19720139
Genes Genet Syst. 2015;90(5):245-57
pubmed: 26781081
J Virol. 2008 Jun;82(11):5295-306
pubmed: 18367535
Nat Rev Genet. 2007 Feb;8(2):93-103
pubmed: 17230196
Front Immunol. 2018 Mar 19;9:546
pubmed: 29616037
Nucleic Acids Res. 2003 Jul 1;31(13):3576-9
pubmed: 12824369
Biomedicines. 2018 Mar 30;6(2):
pubmed: 29601548
Genes Chromosomes Cancer. 2006 Apr;45(4):319-31
pubmed: 16320248
RNA. 2003 Mar;9(3):277-9
pubmed: 12592000
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
Genetics. 2007 Jun;176(2):1323-37
pubmed: 17435244
PLoS One. 2007 Feb 14;2(2):e203
pubmed: 17301878
FEBS Lett. 2012 Jul 30;586(16):2382-8
pubmed: 22677169
PLoS Biol. 2003 Dec;1(3):E60
pubmed: 14691535
Biomed Pharmacother. 2018 Jun;102:203-211
pubmed: 29558717
Elife. 2015 Aug 12;4:
pubmed: 26267216