Quantifying the effects of antibiotic treatment on the extracellular polymer network of antimicrobial resistant and sensitive biofilms using multiple particle tracking.
Biofilms
/ drug effects
Dose-Response Relationship, Drug
Drug Resistance, Bacterial
Escherichia coli
/ drug effects
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Microbial Sensitivity Tests
Microbial Viability
/ drug effects
Microscopy, Confocal
Nanoparticles
Particle Size
Polymyxin B
/ pharmacology
Pseudomonas aeruginosa
/ drug effects
Single Molecule Imaging
/ methods
Journal
NPJ biofilms and microbiomes
ISSN: 2055-5008
Titre abrégé: NPJ Biofilms Microbiomes
Pays: United States
ID NLM: 101666944
Informations de publication
Date de publication:
05 02 2021
05 02 2021
Historique:
received:
06
05
2020
accepted:
24
11
2020
entrez:
6
2
2021
pubmed:
7
2
2021
medline:
23
9
2021
Statut:
epublish
Résumé
Novel therapeutics designed to target the polymeric matrix of biofilms requires innovative techniques to accurately assess their efficacy. Here, multiple particle tracking (MPT) was developed to characterize the physical and mechanical properties of antimicrobial resistant (AMR) bacterial biofilms and to quantify the effects of antibiotic treatment. Studies employed nanoparticles (NPs) of varying charge and size (40-500 nm) in Pseudomonas aeruginosa PAO1 and methicillin-resistant Staphylococcus aureus (MRSA) biofilms and also in polymyxin B (PMB) treated Escherichia coli biofilms of PMB-sensitive (PMB
Identifiants
pubmed: 33547326
doi: 10.1038/s41522-020-00172-6
pii: 10.1038/s41522-020-00172-6
pmc: PMC7864955
doi:
Substances chimiques
Polymyxin B
J2VZ07J96K
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13Subventions
Organisme : Medical Research Council
ID : MC_PC_17186
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S013768/2
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC-PC_17186
Pays : United Kingdom
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