Measurement of Melanin Metabolism in Live Cells by [U-

Adenylyl Cyclases / genetics Animals Carbon Isotopes / analysis Cells, Cultured Chromatography, High Pressure Liquid / methods Humans Mass Spectrometry / methods Melanins / analysis Melanosomes / metabolism Mice Mice, Knockout Primary Cell Culture Receptor, Melanocortin, Type 1 / genetics Skin Pigmentation Tyrosine / analysis

Journal

The Journal of investigative dermatology
ISSN: 1523-1747
Titre abrégé: J Invest Dermatol
Pays: United States
ID NLM: 0426720

Informations de publication

Date de publication:
07 2021
Historique:
received: 31 08 2020
revised: 17 12 2020
accepted: 20 01 2021
pubmed: 8 2 2021
medline: 24 11 2021
entrez: 7 2 2021
Statut: ppublish

Résumé

Melanin synthesis occurs within a specialized organelle called the melanosome. Traditional methods for measuring melanin levels rely on the detection of chemical degradation products of melanin by high-performance liquid chromatography. Although these methods are robust, they are unable to distinguish between melanin synthesis and degradation and are best suited to measure melanin changes over long periods of time. We developed a method that actively measures both eumelanin and pheomelanin synthesis by fate tracing [U-

Identifiants

DOI: 10.1016/j.jid.2021.01.007 PMID: 33549605 PMC: PMC8830938
pubmed: 33549605
pii: S0022-202X(21)00078-6
doi: 10.1016/j.jid.2021.01.007
pmc: PMC8830938
mid: NIHMS1670219
pii:
doi:

Substances chimiques

Carbon Isotopes 0
MC1R protein, human 0
Melanins 0
Receptor, Melanocortin, Type 1 0
pheomelanin 0
eumelanin 12627-86-0
Tyrosine 42HK56048U
Adenylyl Cyclases EC 4.6.1.1
Carbon-13 FDJ0A8596D

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

1810-1818.e6

Subventions

Organisme : NIAMS NIH HHS
ID : R01 AR077664
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR076029
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA224391
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : BLRD VA
ID : I01 BX003668
Pays : United States
Organisme : NIEHS NIH HHS
ID : R21 ES032347
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007739
Pays : United States
Organisme : Wellcome Trust
ID : 108429/Z/15/Z
Pays : United Kingdom

Informations de copyright

Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

Références

Int J Biochem Cell Biol. 2016 Dec;81(Pt B):375-382
pubmed: 27732890
Pigment Cell Melanoma Res. 2011 Feb;24(1):63-74
pubmed: 20726950
Photochem Photobiol. 2008 Mar-Apr;84(2):501-8
pubmed: 18282187
FASEB J. 2010 Oct;24(10):3850-60
pubmed: 20519635
Pigment Cell Melanoma Res. 2014 Sep;27(5):699-720
pubmed: 24807163
Experientia. 1977 Aug 15;33(8):1118-9
pubmed: 408183
Cells. 2020 Apr 08;9(4):
pubmed: 32276449
Pigment Cell Res. 2001 Feb;14(1):23-31
pubmed: 11277491
Nature. 2006 Sep 21;443(7109):340-4
pubmed: 16988713
Pigment Cell Melanoma Res. 2009 Oct;22(5):635-44
pubmed: 19558415
Physiology (Bethesda). 2012 Apr;27(2):85-99
pubmed: 22505665
PLoS One. 2015 Dec 16;10(12):e0143937
pubmed: 26674646
Pigment Cell Res. 2002 Jun;15(3):174-83
pubmed: 12028581
Cell Rep. 2017 Nov 14;21(7):1795-1808
pubmed: 29141214
Oxid Med Cell Longev. 2014;2014:860479
pubmed: 24790705
Pigment Cell Res. 2000 Aug;13(4):273-7
pubmed: 10952395
Exp Cell Res. 2001 Jan 15;262(2):197-208
pubmed: 11139343
J Biol Chem. 1991 Sep 15;266(26):17707-12
pubmed: 1832676
Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14785-9
pubmed: 17001010
FASEB J. 2006 Sep;20(11):1927-9
pubmed: 16877522
J Invest Dermatol. 2013 Oct;133(10):2416-2424
pubmed: 23558403
FASEB J. 2003 Jul;17(10):1248-55
pubmed: 12832289
Methods Enzymol. 2004;378:88-109
pubmed: 15038959
Int J Mol Sci. 2013 Jun 07;14(6):12222-48
pubmed: 23749111
EMBO J. 1997 Jun 16;16(12):3544-52
pubmed: 9218796
Korean J Pediatr. 2014 Jan;57(1):1-18
pubmed: 24578711
PLoS One. 2015 Jun 09;10(6):e0129273
pubmed: 26057890
Pigment Cell Melanoma Res. 2015 Sep;28(5):520-44
pubmed: 26176788
Pigment Cell Melanoma Res. 2021 Jan;34(1):28-43
pubmed: 32777162
Sci Signal. 2018 Nov 06;11(555):
pubmed: 30401788
Pigment Cell Melanoma Res. 2020 May;33(3):403-415
pubmed: 31659857
Cell Mol Life Sci. 2018 Oct;75(20):3803-3815
pubmed: 29705952
Elife. 2014 Dec 16;3:e04543
pubmed: 25513726
Int J Mol Sci. 2017 Dec 22;19(1):
pubmed: 29271951
Pigment Cell Res. 2003 Oct;16(5):523-31
pubmed: 12950732
Photochem Photobiol. 2008 May-Jun;84(3):582-92
pubmed: 18435614
Pigment Cell Melanoma Res. 2017 May;30(3):372-377
pubmed: 28271633
Gene. 2001 Oct 17;277(1-2):49-62
pubmed: 11602344
PLoS One. 2020 Sep 17;15(9):e0239019
pubmed: 32941497
Proc Natl Acad Sci U S A. 2006 Oct 3;103(40):14647-8
pubmed: 17005730
Peptides. 2007 Dec;28(12):2387-96
pubmed: 18006116
Pigment Cell Melanoma Res. 2019 Nov;32(6):854-863
pubmed: 31233279
Cell Metab. 2018 May 01;27(5):1007-1025.e5
pubmed: 29657030
Redox Biol. 2020 May;32:101472
pubmed: 32171726
Nature. 2012 Nov 15;491(7424):449-53
pubmed: 23123854
Cell. 2012 Oct 12;151(2):372-83
pubmed: 23063126
PLoS One. 2014 Aug 05;9(8):e104367
pubmed: 25093503
Front Cell Neurosci. 2015 Mar 17;9:86
pubmed: 25852478
EMBO J. 2014 Mar 3;33(5):501-11
pubmed: 24502975
PLoS One. 2012;7(6):e37149
pubmed: 22723833
J Biol Chem. 1998 Nov 27;273(48):31801-5
pubmed: 9822646
Photochem Photobiol Sci. 2013 Jan;12(1):65-77
pubmed: 23123922
Curr Biol. 2014 Feb 17;24(4):393-403
pubmed: 24485836
Biochim Biophys Acta Mol Basis Dis. 2017 Oct;1863(10 Pt A):2448-2461
pubmed: 28259754
J Invest Dermatol. 2010 Feb;130(2):520-8
pubmed: 19710684
Pigment Cell Res. 2006 Apr;19(2):154-62
pubmed: 16524431
J Invest Dermatol. 2012 May;132(5):1452-61
pubmed: 22336944
Biochim Biophys Acta. 1997 Oct 20;1336(3):539-48
pubmed: 9367182
Biochim Biophys Acta Biomembr. 2020 Dec 1;1862(12):183318
pubmed: 32333855
Exp Cell Res. 2004 Aug 15;298(2):521-34
pubmed: 15265699
Hum Mutat. 2008 Sep;29(9):1161-7
pubmed: 18563784
Exp Dermatol. 2008 May;17(5):395-404
pubmed: 18177348
Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10218-23
pubmed: 22615359
J Dermatol Sci. 2013 Jan;69(1):30-7
pubmed: 23103111
J Gen Physiol. 2016 Oct;148(4):325-39
pubmed: 27670898
Exp Cell Res. 2001 Aug 1;268(1):26-35
pubmed: 11461115
Arch Biochem Biophys. 2014 Dec 1;563:35-41
pubmed: 25034214
J Physiol. 2007 Feb 1;578(Pt 3):641-53
pubmed: 17138610
Proc Natl Acad Sci U S A. 2016 May 17;113(20):5622-7
pubmed: 27140606
Int J Biochem Cell Biol. 2010 Jul;42(7):1094-104
pubmed: 20381640
Development. 1989 Feb;105(2):379-85
pubmed: 2806130
Am J Hum Biol. 2010 Jul-Aug;22(4):526-37
pubmed: 20209486

Auteurs

Qiuying Chen (Q)

Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, USA.

Dalee Zhou (D)

Department of Dermatology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, USA.

Zalfa Abdel-Malek (Z)

Department of Dermatology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.

Fengli Zhang (F)

Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, USA.

Philip S Goff (PS)

Cell Biology Research Centre, Molecular and Clinical Sciences Research Institute, St George's University of London, London, United Kingdom.

Elena V Sviderskaya (EV)

Cell Biology Research Centre, Molecular and Clinical Sciences Research Institute, St George's University of London, London, United Kingdom.

Kazumasa Wakamatsu (K)

Institute for Melanin Chemistry, Fujita Health University, Toyoake, Japan.

Shosuke Ito (S)

Institute for Melanin Chemistry, Fujita Health University, Toyoake, Japan.

Steven S Gross (SS)

Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, USA.

Jonathan H Zippin (JH)

Department of Pharmacology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, USA; Department of Dermatology, Joan and Sanford I. Weill Medical College of Cornell University, New York, New York, USA. Electronic address: jhzippin@med.cornell.edu.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Adenylate Cyclase › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Eucaryotes › Animaux › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Produits chimiques inorganiques › Isotopes › Isotopes du carbone › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Cellules › Cellules cultivées › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Mélanines › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Cellules › Cellules épithéliales › Mélanocytes › Mélanosomes › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Souris knockout › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur hormone hypophysaire › Récepteur corticotrophine › Récepteurs à la mélanocortine › Récepteur de la mélanocortine de type 1 › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Phénomènes biologiques › Pigmentation › Pigmentation de la peau › Measurement of Melanin Metabolism in Live Cells by [U-
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés cycliques › Acides aminés aromatiques › Tyrosine › Measurement of Melanin Metabolism in Live Cells by [U-