Incorporation of sensing modalities into de novo designed fluorescence-activating proteins.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
08 02 2021
08 02 2021
Historique:
received:
29
06
2020
accepted:
10
08
2020
entrez:
9
2
2021
pubmed:
10
2
2021
medline:
17
2
2021
Statut:
epublish
Résumé
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based on green fluorescent protein and its relatives have been engineered in recent years. Here we explore the incorporation of sensing modalities into de novo designed fluorescence-activating proteins, called mini-fluorescence-activating proteins (mFAPs), that bind and stabilize the fluorescent cis-planar state of the fluorogenic compound DFHBI. We show through further design that the fluorescence intensity and specificity of mFAPs for different chromophores can be tuned, and the fluorescence made sensitive to pH and Ca
Identifiants
pubmed: 33558528
doi: 10.1038/s41467-020-18911-w
pii: 10.1038/s41467-020-18911-w
pmc: PMC7870846
doi:
Substances chimiques
Fluorescent Dyes
0
Luminescent Proteins
0
Green Fluorescent Proteins
147336-22-9
Acetylcholine
N9YNS0M02X
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
856Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
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