High-mobility group box-1 and inter-alpha inhibitor proteins: In vitro binding and co-localization in cerebral cortex after hypoxic-ischemic injury.
brain injury
high-mobility group box 1
hypoxia-ischemia
inter-alpha inhibitor proteins
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
09
09
2020
revised:
09
01
2021
accepted:
12
01
2021
entrez:
9
2
2021
pubmed:
10
2
2021
medline:
27
7
2021
Statut:
ppublish
Résumé
The high-mobility group box-1 (HMGB1) protein is a transcription-regulating protein located in the nucleus. However, it serves as a damage-associated molecular pattern protein that activates immune cells and stimulates inflammatory cytokines to accentuate neuroinflammation after release from damaged cells. In contrast, Inter-alpha Inhibitor Proteins (IAIPs) are proteins with immunomodulatory effects including inhibition of pro-inflammatory cytokines. We have demonstrated that IAIPs exhibit neuroprotective properties in neonatal rats exposed to hypoxic-ischemic (HI) brain injury. In addition, previous studies have suggested that the light chain of IAIPs, bikunin, may exert its anti-inflammatory effects by inhibiting HMGB1 in a variety of different injury models in adult subjects. The objectives of the current study were to confirm whether HMGB1 is a target of IAIPs by investigating the potential binding characteristics of HMGB1 and IAIPs in vitro, and co-localization in vivo in cerebral cortices after exposure to HI injury. Solid-phase binding assays and surface plasmon resonance (SPR) were used to determine the physical binding characteristics between IAIPs and HMGB1. Cellular localizations of IAIPs-HMGB1 in neonatal rat cortex were visualized by double labeling with anti-IAIPs and anti-HMGB1 antibodies. Solid-phase binding and SPR demonstrated specific binding between IAIPs and HMGB1 in vitro. Cortical cytoplasmic and nuclear co-localization of IAIPs and HMGB1 were detected by immunofluorescent staining in control and rats immediately and 3 hours after HI. In conclusion, HMGB1 and IAIPs exhibit direct binding in vitro and co-localization in vivo in neonatal rats exposed to HI brain injury suggesting HMGB1 could be a target of IAIPs.
Identifiants
pubmed: 33559227
doi: 10.1096/fj.202002109RR
doi:
Substances chimiques
Alpha-Globulins
0
HMGB1 Protein
0
Hbp1 protein, rat
0
inter-alpha-inhibitor
39346-44-6
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21399Subventions
Organisme : NINDS NIH HHS
ID : R21 NS095130
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS096525
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD057100
Pays : United States
Organisme : NINDS NIH HHS
ID : R44 NS084575
Pays : United States
Informations de copyright
© 2021 Federation of American Societies for Experimental Biology.
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