A Novel Artificially Humanized Anti-Cripto-1 Antibody Suppressing Cancer Cell Growth.
Amino Acid Sequence
Antibodies, Monoclonal, Humanized
/ immunology
Antineoplastic Agents
/ immunology
Breast Neoplasms
/ drug therapy
Cell Proliferation
Female
GPI-Linked Proteins
/ immunology
Humans
Intercellular Signaling Peptides and Proteins
/ immunology
Male
Neoplasm Proteins
/ immunology
Sequence Homology
Teratocarcinoma
/ drug therapy
Testicular Neoplasms
/ drug therapy
Tumor Cells, Cultured
Cripto-1
anti-Cripto-1 antibody
artificial humanized antibody
cell growth inhibition
phage display library
tissue-micro array
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
08 Feb 2021
08 Feb 2021
Historique:
received:
18
01
2021
accepted:
01
02
2021
entrez:
11
2
2021
pubmed:
12
2
2021
medline:
13
4
2021
Statut:
epublish
Résumé
Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto-1 antibody was produced and purified. The purified antibody showed affinity to recombinant Cripto-1 at 1.1 pmol and immunoreactivity to cancer tissues and cell lines. The antibody was available to detect the immunoreactivity in tissue microarrays of malignant tumors as well as in Cripto-1 overexpressing cells. Simultaneously, the antibody exhibited the potential to suppress the growth of human colon cancer derived GEO cells overexpressing Cripto-1 with IC
Identifiants
pubmed: 33567764
pii: ijms22041709
doi: 10.3390/ijms22041709
pmc: PMC7915030
pii:
doi:
Substances chimiques
Antibodies, Monoclonal, Humanized
0
Antineoplastic Agents
0
GPI-Linked Proteins
0
Intercellular Signaling Peptides and Proteins
0
Neoplasm Proteins
0
TDGF1 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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