Flagellin-based electrochemical sensing layer for arsenic detection in water.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 02 2021
10 02 2021
Historique:
received:
25
09
2020
accepted:
27
01
2021
entrez:
11
2
2021
pubmed:
12
2
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Regular monitoring of arsenic concentrations in water sources is essential due to the severe health effects. Our goal was to develop a rapidly responding, sensitive and stable sensing layer for the detection of arsenic. We have designed flagellin-based arsenic binding proteins capable of forming stable filament structures with high surface binding site densities. The D3 domain of Salmonella typhimurium flagellin was replaced with an arsenic-binding peptide motif of different bacterial ArsR transcriptional repressor factors. We have shown that the fusion proteins developed retain their polymerization ability and have thermal stability similar to that of wild-type filament. The strong arsenic binding capacity of the monomeric proteins was confirmed by isothermal titration calorimetry (ITC), and dissociation constants (K
Identifiants
pubmed: 33568718
doi: 10.1038/s41598-021-83053-y
pii: 10.1038/s41598-021-83053-y
pmc: PMC7876115
doi:
Substances chimiques
Proteins
0
Water
059QF0KO0R
Flagellin
12777-81-0
Gold
7440-57-5
Arsenic
N712M78A8G
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3497Références
ACS Biomater Sci Eng. 2020 Jul 13;6(7):3811-3820
pubmed: 33463317
Environ Sci Technol. 2012 Oct 16;46(20):11213-9
pubmed: 22866936
Biosens Bioelectron. 2015 Jan 15;63:533-545
pubmed: 25150780
Protein Eng Des Sel. 2012 Apr;25(4):153-7
pubmed: 22301275
Nat Struct Biol. 1998 Feb;5(2):125-32
pubmed: 9461078
Rev Sci Instrum. 2014 Jan;85(1):015120
pubmed: 24517825
Biosensors (Basel). 2017 Mar 13;7(1):
pubmed: 28335390
ACS Synth Biol. 2017 Nov 17;6(11):1979-1987
pubmed: 28771313
Biosens Bioelectron. 2017 Sep 15;95:106-116
pubmed: 28431363
Anal Chim Acta. 2012 Jan 13;710:50-7
pubmed: 22123111
J Mol Biol. 2010 Nov 5;403(4):607-15
pubmed: 20868693
Anal Chim Acta. 2007 Mar 7;585(2):312-22
pubmed: 17386680
Nature. 2003 Aug 7;424(6949):643-50
pubmed: 12904785
J Biol Chem. 2007 Nov 23;282(47):34346-55
pubmed: 17897948
J Mol Biol. 2007 Jan 26;365(4):1102-16
pubmed: 17109884
Appl Environ Microbiol. 2019 May 16;85(11):
pubmed: 30952659
Sci Rep. 2018 Feb 26;8(1):3584
pubmed: 29483707
Mol Biotechnol. 2015 Sep;57(9):814-9
pubmed: 25966869
Adv Mater. 2018 Jan;30(4):
pubmed: 29205532
Talanta. 2002 Aug 16;58(1):201-35
pubmed: 18968746
Mikrochim Acta. 2018 Sep 25;185(10):478
pubmed: 30255387
Nat Chem Biol. 2019 May;15(5):540-548
pubmed: 30911179
Lab Chip. 2011 Jul 21;11(14):2369-77
pubmed: 21614381
Annu Rev Earth Planet Sci. 2014 May 1;42:443-467
pubmed: 26778863
Biosensors (Basel). 2014 Nov 25;4(4):494-512
pubmed: 25587436
Nucleic Acids Res. 1990 Feb 11;18(3):619-24
pubmed: 2408017
Nano Lett. 2005 Jun;5(6):1023-7
pubmed: 15943436
Biosens Bioelectron. 2013 Sep 15;47:237-42
pubmed: 23584229
Environ Health Perspect. 2013 Mar;121(3):295-302
pubmed: 23458756
Mol Microbiol. 2017 Nov;106(3):469-478
pubmed: 28861914
Chembiochem. 2016 Nov 3;17(21):2075-2082
pubmed: 27528487
J Struct Biol. 2019 Aug 1;207(2):209-217
pubmed: 31136796