Influence of high glucose in the expression of miRNAs and IGF1R signaling pathway in human myometrial explants.
High glucose
Human
IGF1R signaling pathway
Myometrium
miRNAs
Journal
Archives of gynecology and obstetrics
ISSN: 1432-0711
Titre abrégé: Arch Gynecol Obstet
Pays: Germany
ID NLM: 8710213
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
20
04
2020
accepted:
15
12
2020
pubmed:
13
2
2021
medline:
7
10
2021
entrez:
12
2
2021
Statut:
ppublish
Résumé
Several roles are attributed to the myometrium including sperm and embryo transport, menstrual discharge, control of uterine blood flow, and labor. Although being a target of diabetes complications, the influence of high glucose on this compartment has been poorly investigated. Both miRNAs and IGF1R are associated with diabetic complications in different tissues. Herein, we examined the effects of high glucose on the expression of miRNAs and IGF1R signaling pathway in the human myometrium. Human myometrial explants were cultivated for 48 h under either high or low glucose conditions. Thereafter, the conditioned medium was collected for biochemical analyses and the myometrial samples were processed for histological examination as well as miRNA and mRNA expression profiling by qPCR. Myometrial structure and morphology were well preserved after 48 h of cultivation in both high and low glucose conditions. Levels of lactate, creatinine, LDH and estrogen in the supernatant were similar between groups. An explorative screening by qPCR arrays revealed that 6 out of 754 investigated miRNAs were differentially expressed in the high glucose group. Data validation by single qPCR assays confirmed diminished expression of miR-215-5p and miR-296-5p, and also revealed reduced miR-497-3p levels. Accordingly, mRNA levels of IGF1R and its downstream mediators FOXO3 and PDCD4, which are potentially targeted by miR-497-3p, were elevated under high glucose conditions. In contrast, mRNA expression of IGF1, PTEN, and GLUT1 was unchanged. The human myometrium responds to short-term exposure (48 h) to high glucose concentrations by regulating the expression of miRNAs, IGF1R and its downstream targets.
Identifiants
pubmed: 33575847
doi: 10.1007/s00404-020-05940-5
pii: 10.1007/s00404-020-05940-5
pmc: PMC8087607
doi:
Substances chimiques
Apoptosis Regulatory Proteins
0
IGF1R protein, human
0
MIRN296 microRNA, human
0
MIRN497 microRNA, human
0
MicroRNAs
0
PDCD4 protein, human
0
RNA-Binding Proteins
0
Receptor, IGF Type 1
EC 2.7.10.1
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1513-1522Références
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